An atlas of cell types in the mouse epididymis and vas deferens
Autor: | Manuel Garber, Kyle Gellatly, Onur Yukselen, Oliver J. Rando, Alper Kucukural, Upasna Sharma, Vera D. Rinaldi, Morten Rasmussen, Elisa Donnard |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Male Cell type Stromal cell Mouse QH301-705.5 Science Cell sperm maturation Biology General Biochemistry Genetics and Molecular Biology reproduction 03 medical and health sciences Mice 0302 clinical medicine Immune system Vas Deferens medicine Animals Biology (General) development Epididymis 030219 obstetrics & reproductive medicine General Immunology and Microbiology General Neuroscience Vas deferens Genetics and Genomics General Medicine Cell biology Tools and Resources 030104 developmental biology medicine.anatomical_structure Medicine Single-Cell Analysis Spermatogenesis Developmental biology Developmental Biology |
Zdroj: | eLife eLife, Vol 9 (2020) |
ISSN: | 2050-084X |
Popis: | Following testicular spermatogenesis, mammalian sperm continue to mature in a long epithelial tube known as the epididymis, which plays key roles in remodeling sperm protein, lipid, and RNA composition. To understand the roles for the epididymis in reproductive biology, we generated a single-cell atlas of the murine epididymis and vas deferens. We recovered key epithelial cell types including principal cells, clear cells, and basal cells, along with associated support cells that include fibroblasts, smooth muscle, macrophages and other immune cells. Moreover, our data illuminate extensive regional specialization of principal cell populations across the length of the epididymis. In addition to region-specific specialization of principal cells, we find evidence for functionally specialized subpopulations of stromal cells, and, most notably, two distinct populations of clear cells. Our dataset extends on existing knowledge of epididymal biology, and provides a wealth of information on potential regulatory and signaling factors that bear future investigation. |
Databáze: | OpenAIRE |
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