Anthocyanins Protect Hepatocytes against CCl4-Induced Acute Liver Injury in Rats by Inhibiting Pro-inflammatory mediators, Polyamine Catabolism, Lipocalin-2, and Excessive Proliferation of Kupffer Cells
| Autor: | Zorica Jović, Dušan Sokolović, Aleksandra R. Zarubica, Ljubinka Jankovic Velickovic, Aleksandar Milosavljević, Marko Stojanović, Marko Stanković, Nataša Poklar Ulrih, Goran Radenković, Andrej Veljkovic, Gordana R. Nikolić, Vladimir Petrovic, Violeta Rakić, Dejan B. Popovic, Аzra Lukač, Gordana Kocic, Andrija Jović, Vanja Ničković, Danka Sokolovic, Vuka Katić |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
antioksidanti Antioxidant Physiology Sorbitol dehydrogenase medicine.medical_treatment Clinical Biochemistry Pharmacology Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine anti-inflammatory effects polyamine catabolism medicine kupffer cells Kupffer cells Molecular Biology NADPH oxidase bolezni jeter biology Catabolism Chemistry udc:616.36:577.1:547.973 Glutamate dehydrogenase lipocalin-2 lcsh:RM1-950 antociani Cell Biology Glutathione anthocyanins Nitric oxide synthase Polyamine Catabolism lcsh:Therapeutics. Pharmacology 030104 developmental biology rastlinski izvlečki 030220 oncology & carcinogenesis biology.protein protivnetno delovanje carbon tetrachloride |
| Zdroj: | Antioxidants Volume 8 Issue 10 Antioxidants, vol. 8, no. 10, 451, 2019. Antioxidants, Vol 8, Iss 10, p 451 (2019) |
| ISSN: | 2076-3921 |
| DOI: | 10.3390/antiox8100451 |
| Popis: | This study examined the hepatoprotective and anti-inflammatory effects of anthocyanins from Vaccinim myrtillus (bilberry) fruit extract on the acute liver failure caused by carbon tetrachloride-CCl4 (3 mL/kg, i.p.). The preventive treatment of the bilberry extract (200 mg anthocyanins/kg, orally, 7 days) prior to the exposure to the CCl4 resulted in an evident decrease in markers of liver damage (glutamate dehydrogenase, sorbitol dehydrogenase, malate dehydrogenase), and reduced pro-oxidative (conjugated dienes, lipid hydroperoxide, thiobarbituric acid reactive substances, advanced oxidation protein products, NADPH oxidase, hydrogen peroxide, oxidized glutathione), and pro-inflammatory markers (tumor necrosis factor-alpha, interleukin-6, nitrite, myeloperoxidase, inducible nitric oxide synthase, cyclooxygenase-2, CD68, lipocalin-2), and also caused a significant decrease in the dissipation of the liver antioxidative defence capacities (reduced glutathione, glutathione S-transferase, and quinone reductase) in comparison to the results detected in the animals treated with CCl4 exclusively. The administration of the anthocyanins prevented the arginine metabolism&rsquo s diversion towards the citrulline, decreased the catabolism of polyamines (the activity of putrescine oxidase and spermine oxidase), and significantly reduced the excessive activation and hyperplasia of the Kupffer cells. There was also an absence of necrosis, in regard to the toxic effect of CCl4 alone. The hepatoprotective mechanisms of bilberry extract are based on the inhibition of pro-oxidative mediators, strong anti-inflammatory properties, inducing of hepatic phase II antioxidant enzymes (glutathione S-transferase, quinone reductase) and reduced glutathione, hypoplasia of Kupffer cells, and a decrease in the catabolism of polyamines. |
| Databáze: | OpenAIRE |
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