In vitro selection of RNA aptamers to a small molecule target
| Autor: | Andrew D. Ellington, Sulay D. Jhaveri, Bradley Hall, Vlad Codrea, Michelle Hayner |
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| Rok vydání: | 2008 |
| Předmět: |
Biomedical Research
Aptamer Oligonucleotides DNA Single-Stranded Computational biology Biology Biochemistry Small Molecule Libraries chemistry.chemical_compound Methods Receptor Selection (genetic algorithm) Organic Chemistry SELEX Aptamer Technique RNA General Medicine Aptamers Nucleotide Molecular biology Small molecule In vitro chemistry Nucleic acid DNA Function (biology) Filtration |
| Zdroj: | Current Protocols in Nucleic Acid Chemistry |
| ISSN: | 1934-9289 |
| Popis: | This unit describes the selection of aptamers from a single-stranded RNA pool that bind to small molecule targets. Aptamers generated by this type of selection experiment can potentially function as receptors for small molecules in numerous applications, including medical diagnostics, therapeutics, and environmental monitoring. This unit describes two modes of selection, one by column filtration and one by batch selection. Curr. Protoc. Nucleic Acid Chem. 40:9.5.1-9.5.23. © 2010 by John Wiley & Sons, Inc. Keywords: aptamer; affinity matrix; target immobilization; small molecule |
| Databáze: | OpenAIRE |
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