Space Radiation-Induced Alterations in the Hippocampal Ubiquitin-Proteome System
| Autor: | William K. Russell, Arriyam S Fesshaye, Alyssa Tidmore, Sucharita M. Dutta, Richard A. Britten, Vania D. Duncan |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male Proteomics space radiation cognition medicine.medical_specialty Extraterrestrial Environment QH301-705.5 hippocampus proteome Hippocampus Hippocampal formation Memory performance Catalysis Article Inorganic Chemistry 03 medical and health sciences 0302 clinical medicine Ubiquitin Internal medicine ubiquitin medicine Memory impairment Animals Biology (General) Physical and Theoretical Chemistry Rats Wistar QD1-999 Molecular Biology Spectroscopy biology Proteomic Profiling Organic Chemistry Dose-Response Relationship Radiation General Medicine spatial memory Space radiation Computer Science Applications Rats Chemistry 030104 developmental biology Endocrinology Proteome biology.protein 030217 neurology & neurosurgery Cosmic Radiation |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 14 International Journal of Molecular Sciences, Vol 22, Iss 7713, p 7713 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms22147713 |
| Popis: | Exposure of rodents to < 20 cGy Space Radiation (SR) impairs performance in several hippocampus-dependent cognitive tasks, including spatial memory. However, there is considerable inter-individual susceptibility to develop SR-induced spatial memory impairment. In this study, a robust label-free mass spectrometry (MS)-based unbiased proteomic profiling approach was used to characterize the composition of the hippocampal proteome in adult male Wistar rats exposed to 15 cGy of 1 GeV/n 48Ti and their sham counterparts. Unique protein signatures were identified in the hippocampal proteome of: (1) sham rats, (2) Ti-exposed rats, (3) Ti-exposed rats that had sham-like spatial memory performance, and (4) Ti-exposed rats that impaired spatial memory performance. Approximately 14% (159) of the proteins detected in hippocampal proteome of sham rats were not detected in the Ti-exposed rats. We explored the possibility that the loss of the Sham-only proteins may arise as a result of SR-induced changes in protein homeostasis. SR-exposure was associated with a switch towards increased pro-ubiquitination proteins from that seen in Sham. These data suggest that the role of the ubiquitin-proteome system as a determinant of SR-induced neurocognitive deficits needs to be more thoroughly investigated. |
| Databáze: | OpenAIRE |
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