Assessment of the molluscicidal impact of extracted chlorophyllin on some biochemical parameters in the nervous tissue and histological changes in Biomphalaria alexandrina and Lymnaea natalensis snails
| Autor: | Amina M. Ibrahim, Fayez A. Bakry |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Fascioliasis Veterinary medicine Molluscacides Snails Schistosomiasis Snail Disease Vectors Biomphalaria alexandrina Moringa 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound 0302 clinical medicine Developmental Neuroscience biology.animal parasitic diseases medicine Animals Fasciolosis Chlorophyllides biology Plant Extracts Chlorophyllin Acid phosphatase Intermediate host medicine.disease biology.organism_classification 030104 developmental biology chemistry biology.protein 030217 neurology & neurosurgery |
| Zdroj: | Invertebrate Neuroscience. 19 |
| ISSN: | 1439-1104 1354-2516 |
| DOI: | 10.1007/s10158-019-0230-1 |
| Popis: | Biomphalaria alexandrina and Lymnaea natalensis snails are the intermediate hosts of schistosomiasis and fasciolosis. The aim of the present study is to evaluate the molluscicidal activity of chlorophyll extract as a photodynamic substance against these snails and how it affected its tissues and the biological system. Chlorophyllin was extracted from deep-frozen Moringa oleifera leaves, and then it was transformed into water-soluble chlorophyllin. The present results showed that it had a molluscicidal activity on B. alexandrina snails (LC50 17.6 mg/l; LC90 20.9 mg/l) and L. natalensis snails (LC50 4.3 mg/l; LC90 6.8 mg/l). Exposing B. alexandrina snails to the sublethal concentrations (LC0, LC10, and LC25) resulted in a significant reduction in their survival rates. Regarding its effect on biochemical parameters, chlorophyllin significantly reduced the acetylcholinesterase activity, protein content, and alkaline and acid phosphatase activity in B. alexandrina nervous tissue compared to the control group. Histopathological changes occurred in the digestive gland of treated B. alexandrina snails where cells lost their nuclei, vacuolated, degenerated, and ruptured, and the lumen increased. Photosynthesizing materials like chlorophyllin are new approaches to control schistosomiasis and fasciolosis in developing countries by affecting their intermediate host. These materials were cheap and environmentally safe to replace the synthetic molluscicides for snail control. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|