Inhibition of Pro-inflammatory Secreted Phospholipase A2 by Extracts from Cynara cardunculus L
| Autor: | Sofiane Bezzine, Yassine Ben Ali, Mohamed Damak, Imed Koubaa, Raoudha Jarraya, Maher Kammoun, Youssef Gargouri |
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| Rok vydání: | 2009 |
| Předmět: |
medicine.drug_class
Anti-Inflammatory Agents Cynara Bioengineering Fractionation Phospholipase Applied Microbiology and Biotechnology Biochemistry Anti-inflammatory Acetone chemistry.chemical_compound Phospholipase A2 Phospholipase A1 medicine Hexanes Potency Enzyme Inhibitors Phospholipases A2 Secretory Molecular Biology Chromatography biology Plant Extracts Methanol General Medicine biology.organism_classification Enzyme Activation chemistry biology.protein Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 162:662-670 |
| ISSN: | 1559-0291 0273-2289 |
| Popis: | The aim of the present work was to evaluate the anti-inflammatory properties of Cynara cardunculus L. (Asteraceae) during its growth using various solvents such as n-hexane, dichloromethane, acetone, and methanol for air-dried leaves and stems. The anti-inflammatory activities of crude extracts were evaluated by measuring the inhibition potency of mammalian non-pancreatic phospholipases A2 (hG-IIA). The methanol and acetone extracts of leaves harvested in February exhibit potent inhibition of hG-IIA (IC(50) = 50 and 70 microg/ml, respectively). However, the acetone extract of stems harvested in December inhibits the hG-IIA with a lower IC(50) around 130 microg/ml. Fractionation on silica gel and hydrophobic gel of the methanol extract of leaves harvested in February increases the inhibitory effect, and the IC(50) reached 10 microg/ml. |
| Databáze: | OpenAIRE |
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