Expression of parathyroid hormone-related peptide messenger ribonucleic acid in developing kidney
| Autor: | Youjirou Ichinose, Megumi Kobayashi, Kunihiko Aya, Hiroyuki Tanaka, Yoshiki Seino |
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| Rok vydání: | 1999 |
| Předmět: |
Male
musculoskeletal diseases medicine.medical_specialty Renal glomerulus medicine.medical_treatment Kidney Glomerulus Parathyroid hormone Biology Polymerase Chain Reaction Mice Paracrine signalling Internal medicine medicine Animals RNA Messenger Urothelium Growth Substances Autocrine signalling glomerular development In Situ Hybridization DNA Primers Mice Inbred BALB C Kidney urogenital system Growth factor Parathyroid Hormone-Related Protein Gene Expression Regulation Developmental Proteins hypercalcemia growth factor neonates PTH related peptide Endocrinology medicine.anatomical_structure Parathyroid Hormone Nephrology Ureteric bud kidney maturation hormones hormone substitutes and hormone antagonists |
| Zdroj: | Kidney International. 55:1696-1703 |
| ISSN: | 0085-2538 |
| Popis: | Expression of parathyroid hormone-related peptide messenger ribonucleic acid in developing kidney. Background Parathyroid hormone (PTH)-related peptide (PTHrP), originally identified as a causative agent of hypercalcemia of malignancy, has been implicated in the regulation of growth and differentiation of endochondral bone, hair follicle, and breast as an autocrine/paracrine factor. Although some experiments indicate that PTHrP works as a growth factor for primary renal cells in vitro , the role of PTHrP in the kidney in vivo is not yet known. Methods We examined the amounts of PTHrP and PTH/PTHrP receptor (PTHR) mRNA in the mouse kidney developmental process by reverse transcription-polymerase chain reaction, and investigated which cells produce PTHrP and PTHR in vivo by in situ hybridization. Results We observed high levels of PTHrP mRNA during mouse kidney maturation. PTHrP mRNA was expressed in the collecting duct, urothelium of the pelvis, and immature elements in the cortex of the developing kidney, including the S-shaped body, ureteric bud, and glomerulus. However, the expression of PTHR mRNA was lower during maturation than after the completion of the maturation process, and it was not detected in the collecting duct, urothelium of the pelvis, or nephrogenic zone in embryonic day 16 or 0-day-old mouse kidneys. Conclusion These findings suggest that PTHrP has a role in mouse kidney maturation or glomerular development. |
| Databáze: | OpenAIRE |
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