A rapid and simple procedure for the isolation and cultivation of fibroblast-like cells from medaka and zebrafish embryos and fin clip biopsies
| Autor: | Jochen Wittbrodt, Thomas Thumberger, Christian Thiel, Sevinç Gücüm, Andreas Hüllen, Lars Beedgen |
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| Rok vydání: | 2022 |
| Předmět: |
Oryzias
Biopsy ved/biology.organism_classification_rank.species Danio Context (language use) Mice 610 Medical sciences Medicine Japanese rice fish Animals Humans Model organism Zebrafish General Veterinary biology ved/biology fungi Embryo Fibroblasts biology.organism_classification Surgical Instruments Cell biology Rats Cell culture Animal Science and Zoology |
| DOI: | 10.11588/heidok.00031793 |
| Popis: | In many human diseases, the molecular pathophysiological mechanisms are not understood, which makes the development and testing of new therapeutic approaches difficult. The generation and characterization of animal models such as mice, rats, fruit flies, worms or fish offers the possibility for in detail studies of a disease’s development, its course and potential therapies in an organismal context, which considerably minimizes the risk of therapeutic side effects for patients. Nevertheless, due to the high numbers of experimental animals used in research worldwide, attempts to develop alternative test systems will help in reducing their count. In this regard, the cell culture system displays a suitable option due to its potential of delivering nearly unlimited material and the good opportunities for high-throughput studies such as drug testing. Here, we describe a quick and simple method to isolate and cultivate vital fibroblast-like cells from embryos and adults of two popular teleost model organisms, the Japanese rice fish medaka ( Oryzias latipes) and the zebrafish ( Danio rerio). |
| Databáze: | OpenAIRE |
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