Preclinical studies with JAA-F11 anti-Thomsen-Friedenreich monoclonal antibody for human breast cancer
| Autor: | Julia Abdullah, Susan Morey, Jing Ying Eng, Stephen T. Koury, Kimiko Ferguson, Gabriel Hrysenko, Kate Rittenhouse-Olson, Arti Yadav, Munawwar Sajjad |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cancer Research
medicine.drug_class medicine.medical_treatment Mice Nude Antineoplastic Agents Triple Negative Breast Neoplasms Mice SCID Monoclonal antibody Metastasis Mice Breast cancer Cell Line Tumor Animals Humans Medicine Cell Proliferation Bladder cancer biology Thomsen-Friedenreich Antigen business.industry Antibodies Monoclonal Cancer General Medicine Immunotherapy medicine.disease Xenograft Model Antitumor Assays Molecular biology Oncology Cancer research biology.protein Female Antibody business |
| Zdroj: | Future Oncology. 10:385-399 |
| ISSN: | 1744-8301 1479-6694 |
| DOI: | 10.2217/fon.13.209 |
| Popis: | ABSTRACT: Aim: The Thomsen-Friedenreich antigen (TF-Ag) is a disaccharide hidden on normal cells, but selectively exposed on the surface of breast, colon, prostate and bladder cancer cells. JAA-F11, a highly specific monoclonal antibody to TF-Ag, reduces metastasis and prolongs survival in a mouse model. In addition,124I-JAA-F11 localizes 4T1 tumors in mice. These studies continue translation of JAA-F11 to human breast cancer. Materials & methods & results: Of the 41 human breast cancer cell lines tested, 78% were positive for reactivity with JAA-F11 by whole-cell enzyme immunoassay and positivity occurred unrelated to estrogen, progesterone or HER2 receptor status. JAA-F11 inhibited the growth rate of the human cancer cell lines tested. At 1 h, approximately 80% of JAA-F11 internalized in the three cell lines tested. 124I-JAA-F11 specifically imaged human triple-negative tumors in mice by microPET. Conclusion: The results highlight the potential that humanized JAA-F11 may have for immunotherapy and drug conjugate therapy in breast cancer patients. |
| Databáze: | OpenAIRE |
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