Identification of Unprecedented Anticancer Properties of High Molecular Weight Biomacromolecular Complex Containing Bovine Lactoferrin (HMW-bLf)
| Autor: | Sneha Gurudevan, Jagat R. Kanwar, Jayanth Suryanarayanan Shankaranarayanan, Uma Maheswari Krishnan, Rupinder K. Kanwar, Fawzi Ebrahim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Drug Research and Development
media_common.quotation_subject Agricultural Biotechnology lcsh:Medicine Antineoplastic Agents Apoptosis Breast Neoplasms Biochemistry Cell Line Tumor Molecular Cell Biology Drug Discovery Medicine and Health Sciences Animals Humans Internalization Cytotoxicity lcsh:Science media_common Cell Proliferation Pharmacology Multidisciplinary biology Cell growth Lactoferrin Caspase 3 lcsh:R Biology and Life Sciences Proteins Agriculture Cell Biology Actins Up-Regulation Targeted drug delivery Oncology Cell culture Cancer cell Immunology Colonic Neoplasms Dietary Supplements biology.protein Colostrum lcsh:Q Plant Biotechnology Cattle Clinical Medicine Research Article Biotechnology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 9, Iss 9, p e106568 (2014) |
| ISSN: | 1932-6203 |
| Popis: | With the successful clinical trials, multifunctional glycoprotein bovine lactoferrin is gaining attention as a safe nutraceutical and biologic drug targeting cancer, chronic-inflammatory, viral and microbial diseases. Interestingly, recent findings that human lactoferrin oligomerizes under simulated physiological conditions signify the possible role of oligomerization in the multifunctional activities of lactoferrin molecule during infections and in disease targeting signaling pathways. Here we report the purification and physicochemical characterization of high molecular weight biomacromolecular complex containing bovine lactoferrin (≥250 kDa), from bovine colostrum, a naturally enriched source of lactoferrin. It showed structural similarities to native monomeric iron free (Apo) lactoferrin (∼78-80 kDa), retained anti-bovine lactoferrin antibody specific binding and displayed potential receptor binding properties when tested for cellular internalization. It further displayed higher thermal stability and better resistance to gut enzyme digestion than native bLf monomer. High molecular weight bovine lactoferrin was functionally bioactive and inhibited significantly the cell proliferation (p |
| Databáze: | OpenAIRE |
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