Periplasm-enriched fractions from Xanthomonas citri subsp. citri type A and X. fuscans subsp. aurantifolii type B present distinct proteomic profiles under in vitro pathogenicity induction

Autor: Leandro Seiji Goto, José Belasque, Silvia Palma Ferreira, Carolina Moretto Carnielli, Maria Teresa Marques Novo-Mansur, Juliana Artier, Evandro L. Prieto, Nicole C. S. Nicolela, André Vessoni Alexandrino, Flávia S. Zandonadi, Mariana P. Barcelos
Rok vydání: 2020
Předmět:
Proteomics
Cell Membranes
Pathogenesis
Pathology and Laboratory Medicine
Biochemistry
Xanthomonas citri
Medicine and Health Sciences
Peptide sequence
Multidisciplinary
Xylose
medicine.diagnostic_test
biology
Organic Compounds
Monosaccharides
Software Engineering
Genomics
Chemistry
Proteome
Citrus canker
Physical Sciences
Periplasm
Medicine
Engineering and Technology
Phosphoglucomutase
Cellular Structures and Organelles
Research Article
Computer and Information Sciences
Xanthomonas
Science
Carbohydrates
CANCRO (DOENÇA DE PLANTA)
Microbiology
Computer Software
Western blot
Bacterial Proteins
medicine
Genetics
Amino Acid Sequence
Gene Prediction
Organic Chemistry
Chemical Compounds
Biology and Life Sciences
Membrane Proteins
Proteins
Computational Biology
Reproducibility of Results
Molecular Sequence Annotation
Periplasmic space
Cell Biology
biology.organism_classification
Outer Membrane Proteins
Genome Analysis
Carbon
Chaperone Proteins
Genes
Bacterial

Bacteria
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
PLoS ONE
PLoS ONE, Vol 15, Iss 12, p e0243867 (2020)
Popis: The causative agent of Asiatic citrus canker, the Gram-negative bacterium Xanthomonas citri subsp. citri (XAC), produces more severe symptoms and attacks a larger number of citric hosts than Xanthomonas fuscans subsp. aurantifolii XauB and XauC, the causative agents of cancrosis, a milder form of the disease. Here we report a comparative proteomic analysis of periplasmic-enriched fractions of XAC and XauB in XAM-M, a pathogenicity- inducing culture medium, for identification of differential proteins. Proteins were resolved by two-dimensional electrophoresis combined with liquid chromatography-mass spectrometry. Among the 12 proteins identified from the 4 unique spots from XAC in XAM-M (p
Databáze: OpenAIRE