GPETAFLR, a novel bioactive peptide from Lupinus angustifolius L. protein hydrolysate, reduces osteoclastogenesis
| Autor: | Antonio Carrillo-Vico, Sergio Montserrat-de la Paz, M. Mar Yust, Justo Pedroche, Ana Lemus-Conejo, Francisco Millán, M. Carmen Millan-Linares |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), [Carmen Milian-Linares, M.] CSIC, Inst Grasa, Ctra Utrera Km 1, Seville 41013, Spain, [Mar Yust, M.] CSIC, Inst Grasa, Ctra Utrera Km 1, Seville 41013, Spain, [Pedroche, Justo] CSIC, Inst Grasa, Ctra Utrera Km 1, Seville 41013, Spain, [Milian, Francisco] CSIC, Inst Grasa, Ctra Utrera Km 1, Seville 41013, Spain, [Lemus-Conejo, Ana] Univ Seville, Sch Med, Med Biochem Mol Biol & Immunol, Av Dr Fedriani 3, Seville 41071, Spain, [Carrillo-Vico, Antonio] Univ Seville, Sch Med, Med Biochem Mol Biol & Immunol, Av Dr Fedriani 3, Seville 41071, Spain, [Montserrat-de la Paz, Sergio] Univ Seville, Sch Med, Med Biochem Mol Biol & Immunol, Av Dr Fedriani 3, Seville 41071, Spain, [Carrillo-Vico, Antonio] Univ Seville, CSIC, HUVR, Inst Biomed Sevilla,IBiS,Junta Andalucia, Av Manuel Siurot S-N, Seville 41013, Spain, Spanish Ministry of Economy and Competitiveness, 'V Own Research Plan' (University of Seville) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Medicine (miscellaneous) Peptide Hydrolysate Lupine 03 medical and health sciences Bioactive peptide 0302 clinical medicine Osteoclast medicine TX341-641 Protein hydrolysates Bone Gene Inhibition chemistry.chemical_classification Nutrition and Dietetics biology Chemistry Nutrition. Foods and food supply Protein hydrolysate biology.organism_classification Lupinus angustifolius 030104 developmental biology medicine.anatomical_structure Biochemistry Rankl/opg 030220 oncology & carcinogenesis Osteoporosis Food Science Pathway |
| Zdroj: | Journal of Functional Foods, Vol 47, Iss, Pp 299-303 (2018) Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1756-4646 |
| Popis: | 20 Páginas.-- 2 Figuras.-- 1 Tabla The effect of GPETAFLR, a peptide isolated from Lupinus angustifolius L. protein hydrolysate (LPH), on osteoclastogenesis was investigated. Human osteoclasts generated from monocytes were used to analyse the effects of GPETAFLR (50–100 µg/mL) on osteoclastogenesis using TRAP reaction, RT-qPCR, and ELISA procedures. LPS enhanced TRAP activity and the expression of osteoclast marker genes (TRAP, OSCAR, RANK, and CATHK) while downregulated the expression of OPG gene in human monocyte-derived osteoclasts. These effects were reduced with GPETAFLR. Moreover, LPS increased the release of osteoclastogenic cytokines (TNF-α, IL-1β, and IL-6) meanwhile GPETAFLR increased the release of anti-osteoclastogenic cytokines (IL-4 and IL-10) in the medium of human monocyte-derived osteoclasts. For the first time, we show that plant peptides from lupine protein hydrolysates have anti-osteoclastogenic activity. These exciting findings open opportunities for developing nutritional strategies with Lupinus angustifolius L. as dietary source of plant proteins, notably GPETAFLR, to prevent development and progression of osteoclast-related diseases. This work was supported by grant AGL2012-40247-C02-01 from the Spanish Ministry of Economy and Competitiveness. Authors thank Cell Biology Unit of Instituto de la Grasa for its assistance during the fulfillment of this work. SMP acknowledges financial support from “V Own Research Plan” (University of Seville). |
| Databáze: | OpenAIRE |
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