Comparative In Vitro Evaluation of Antibacterial and Osteogenic Activity of Polysaccharide and Flavonoid Fractions Isolated from the leaves of Saussurea controversa
| Autor: | Larisa Litvinova, Igor A. Khlusov, Elena Avdeeva, I. V. Sukhodolo, Irina Zvereva, Yaroslav Reshetov, Mariia R. Karpova, V. V. Shupletsova, Mikhail V. Belousov, Lyudmila S. Mushtovatova, Ekaterina Porokhova, Artem Guryev, Olga Khaziakhmatova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Arabinose
Staphylococcus aureus growth Rhamnose Flavonoid polysaccharides Pharmaceutical Science Mannose human adipose-derived multipotent mesenchymal stromal cells Polysaccharide Analytical Chemistry lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine lcsh:Organic chemistry Drug Discovery staphylococcus aureus growth Physical and Theoretical Chemistry 030304 developmental biology chemistry.chemical_classification 0303 health sciences Organic Chemistry alizarin red staining Glycoside Biochemistry chemistry saussurea controversa dc (asteraceae) Chemistry (miscellaneous) 030220 oncology & carcinogenesis Galactose flavonoids Molecular Medicine cytotoxicity Saussurea controversa DC (Asteraceae) Quercetin |
| Zdroj: | Molecules Volume 24 Issue 20 Molecules, Vol 24, Iss 20, p 3680 (2019) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules24203680 |
| Popis: | The osteogenic, cytotoxic, and antibacterial activities of polysaccharide (PS-SC) and flavonoid (F-SC) fractions isolated from the leaves extract of Saussurea controversa were studied in vitro. F-SC consists of the five quercetin glycosides in the ratio 2:8:10:1:4, which were isolated from the leaves extract of S. controversa and have been characterized previously. PS-SC was first isolated from the leaves extract of S. controversa and has been described. PS-SC consists in 30 compounds is characterized by a high degree of heterogeneity with a heterogeneity index of 19.74. The Mw and Mn of PS-SC were 108.6 and 5.5 kDa, respectively. Structural fragments are represented by galactose, arabinose, xylose, glucose, uronic acids, mannose, and rhamnose in a 10.1:3.3:2.2:2.1:1.7:0.9:0.5 molar ratio. F-SC as compared with PS-SC showed in vitro microbicidal (50 g/L) and better bacteriostatic (6.25 g/L versus 25 g/L of PS-SC) effects against the 24-h growth of Staphylococcus aureus strain 209 P and a 21-day absence of cytotoxicity on human adipose-derived multipotent mesenchymal stromal cells (hAMMSCs). Both fractions (PS-SC> F-SC) at doses of 10&ndash 50 mg/L stimulated differentiation of hAMMSCs into secreting osteoblasts accompanied by local mineralization of extracellular matrix. These fractions of S. controversa and especially F-SC, might be promising peroral drugs in the complex treatment of bone fractures and for prophylaxis of their infectious complications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |