Light Microscopic Histology of Achilles Tendon Ruptures
| Autor: | Nicola Maffulli, Victoria Barrass, Stanley W. B. Ewen |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Adult
Male Fiber structure Biopsy 0206 medical engineering Physical Therapy Sports Therapy and Rehabilitation 02 engineering and technology Achilles Tendon Statistics Nonparametric 03 medical and health sciences 0302 clinical medicine Vascularity Humans Medicine Orthopedics and Sports Medicine Hyaline Rupture Achilles tendon Chi-Square Distribution Staining and Labeling medicine.diagnostic_test business.industry Bright-field microscopy Histology 030229 sport sciences Anatomy Middle Aged musculoskeletal system 020601 biomedical engineering Tendon medicine.anatomical_structure Female medicine.symptom business |
| Zdroj: | The American Journal of Sports Medicine. 28:857-863 |
| ISSN: | 1552-3365 0363-5465 |
| Popis: | We studied biopsies from the Achilles tendons of patients undergoing open repair for a subcutaneous rupture of their Achilles tendons (27 men, 11 women; mean age, 45.3 13.8 years) and specimens of Achilles tendons from persons with no known tendon ailments (43 men, 3 women; mean age, 64.2 9.7 years). Histologic examination was performed using stained slides that were interpreted using a semiquantitative grading scale assessing fiber structure and arrangement, rounding of the nuclei, regional variations in cellularity, increased vascularity, decreased collagen stainability, hyalinization, and glycosaminoglycan. We gave up to three marks for each of these variables, with 0 being normal and 3 being maximally abnormal. All the histology slides were assessed twice in a blinded manner; the agreement between two readings ranged from 0.56 to 0.87 (kappa statistics). The score of ruptured tendons was significantly greater than the average score of control tendons (20.5 3.6 versus 6.5 2.1), and there was significantly higher degeneration in the ruptured tendons. Nonruptured Achilles tendons, even at an advanced age, and ruptured Achilles tendons are clearly part of two distinct populations. Using these staining techniques, light microscopic degeneration is not a feature of tendons from healthy, older persons. |
| Databáze: | OpenAIRE |
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