Monitoring protein ubiquitination and SUMOylation in real-time by NMR

Autor:	Parag Surana, Ranabir Das, Batul Ismail Habibullah, Vasvi Tripathi
Rok vydání:	2020
Předmět:	Models Molecular Time Factors SUMO protein 02 engineering and technology Catalysis 03 medical and health sciences Ubiquitin Materials Chemistry Humans Nuclear Magnetic Resonance Biomolecular 030304 developmental biology chemistry.chemical_classification 0303 health sciences biology Ubiquitination Metals and Alloys Sumoylation General Chemistry Nuclear magnetic resonance spectroscopy 021001 nanoscience & nanotechnology Ubiquitinated Proteins Protein ubiquitination Surfaces Coatings and Films Electronic Optical and Magnetic Materials Enzyme chemistry Covalent bond Ceramics and Composites biology.protein Biophysics 0210 nano-technology
Zdroj:	Chemical Communications. 56:6735-6738
ISSN:	1364-548X 1359-7345
Popis:	The covalent conjugation of ubiquitin (Ub), known as ubiquitination, is a multi-step reaction involving multiple enzymes. We report a real-time, tag-free method to monitor protein ubiquitination by NMR spectroscopy under physiological conditions. The approach is also applicable for monitoring other ubiquitin-like modifications, and the disassembly of Ub polymers.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::704d719d78c9bda782ef34f471ee2245 https://doi.org/10.1039/d0cc02252g Zobrazit plný text záznamu