Inconsistencies in the red blood cell membrane proteome analysis: generation of a database for research and diagnostic applications
| Autor: | Edit Szabó, Pururawa Mayank Chaubey, Hajnalka Sarankó, Lia Hofstetter, Bernd Roschitzki, Bruno Stieger, Tamás Hegedűs, György Várady, Balázs Sarkadi |
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| Přispěvatelé: | University of Zurich, Hegedűs, Tamás |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Proteome
610 Medicine & health 1100 General Agricultural and Biological Sciences Biology Proteomics computer.software_genre 1710 Information Systems General Biochemistry Genetics and Molecular Biology Protein expression 03 medical and health sciences 0302 clinical medicine 1300 General Biochemistry Genetics and Molecular Biology medicine Humans Databases Protein 030304 developmental biology 0303 health sciences Database Erythrocyte Membrane Membrane Proteins Rbc membrane Biomarker (cell) Red blood cell medicine.anatomical_structure Membrane protein 10199 Clinic for Clinical Pharmacology and Toxicology 030220 oncology & carcinogenesis Original Article Membrane proteome General Agricultural and Biological Sciences computer Information Systems |
| Zdroj: | Database: The Journal of Biological Databases and Curation Database, 2015 Database |
| ISSN: | 1758-0463 |
| Popis: | Based on recent results, the determination of the easily accessible red blood cell (RBC) membrane proteins may provide new diagnostic possibilities for assessing mutations, polymorphisms or regulatory alterations in diseases. However, the analysis of the current mass spectrometry-based proteomics datasets and other major databases indicates inconsistencies—the results show large scattering and only a limited overlap for the identified RBC membrane proteins. Here, we applied membrane-specific proteomics studies in human RBC, compared these results with the data in the literature, and generated a comprehensive and expandable database using all available data sources. The integrated web database now refers to proteomic, genetic and medical databases as well, and contains an unexpected large number of validated membrane proteins previously thought to be specific for other tissues and/or related to major human diseases. Since the determination of protein expression in RBC provides a method to indicate pathological alterations, our database should facilitate the development of RBC membrane biomarker platforms and provide a unique resource to aid related further research and diagnostics. Database, 2015 ISSN:1758-0463 |
| Databáze: | OpenAIRE |
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