Combinations of DEAD box proteins distinguish distinct types of RNA: Protein complexes in neurons
| Autor: | Robyn McAdam, Wayne S. Sossin, Frédérique Badeaux, Vanessa Blandford, Luc DesGroseillers, Linda C. Miller, María del Rayo Sánchez-Carbente |
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| Rok vydání: | 2009 |
| Předmět: |
DEAD box
Recombinant Fusion Proteins RNA transport Biology Cytoplasmic Granules Hippocampus RNA Transport DEAD-box RNA Helicases Rats Sprague-Dawley Cellular and Molecular Neuroscience Protein structure Animals Molecular Biology Cells Cultured Neurons chemistry.chemical_classification Rna protein Novel protein RNA Cell Biology Rats Cell biology Isoenzymes Enzyme nervous system chemistry eIF4A Ribosomes Biomarkers |
| Zdroj: | Molecular and Cellular Neuroscience. 40:485-495 |
| ISSN: | 1044-7431 |
| DOI: | 10.1016/j.mcn.2009.01.007 |
| Popis: | Transport of mRNAs to axons and dendrites in neurons is important for growth, polarization and plasticity. Recent proteomic studies in neurons have identified a number of DEAD box proteins as components of RNA granules. Using DEAD box proteins as markers, we have defined classes of RNA:protein structures present in neurons. In particular, we demonstrate that the conjunction of DEAD box 1 and DEAD box 3 identifies a motile ribosome-containing RNA granule present in both axons and dendrites that is similar to the biochemically isolated RNA granule. Conjunction of DEAD box 1 and the novel protein CGI-99 defines a distinct complex in neurons. Attempts to define a P-body like structure with expression of DEAD box 6 and decapping enzymes suggest that this structure may be more complex in neuronal processes than in other compartments. These studies hint at a great complexity in RNA transport and storage in neuronal processes. |
| Databáze: | OpenAIRE |
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