Transplantation of bone marrow-derived mesenchymal stem cells after regional hepatic irradiation ameliorates thioacetamide-induced liver fibrosis in rats
Autor: | Yun Wang, Hao Chai, Cun-hua Shao, Tianfu Dong, Feng Cheng, Sheng-lin Chen, Yue Yu, Lei Deng |
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Rok vydání: | 2013 |
Předmět: |
Liver Cirrhosis
Male Pathology medicine.medical_specialty Population Bone Marrow Cells Smad Proteins Thioacetamide Mesenchymal Stem Cell Transplantation Rats Sprague-Dawley Transforming Growth Factor beta1 chemistry.chemical_compound Medicine Animals education Stem cell transplantation for articular cartilage repair education.field_of_study medicine.diagnostic_test business.industry Mesenchymal stem cell Rats Transplantation medicine.anatomical_structure chemistry Liver Surgery Bone marrow business Liver function tests Homing (hematopoietic) Signal Transduction |
Zdroj: | The Journal of surgical research. 186(1) |
ISSN: | 1095-8673 |
Popis: | Background Recent studies have demonstrated that bone marrow–derived mesenchymal stem cells (BM-MSCs) can potentially revert liver fibrosis, but it is not known if preparative hepatic irradiation (HIR) contributes to the therapeutic effect of transplanted BM-MSCs. In this study, we investigate the effects of HIR on transplanted BM-MSCs in cirrhotic rats and the underlying mechanism by which mesenchymal stem cells (MSCs) relieve liver fibrosis. Materials and methods The BM-MSCs from male rats were labeled with CM-Dil and injected via portal vein into two groups of thioacetamide-induced cirrhotic rats, and the controls were injected with the same volume of saline. The right hemiliver of one cirrhotic rat group was irradiated (15 Gy) 4 d before transplantation. Liver function tests and histologic experiments were performed, and the liver population of BM-MSCs was estimated. Results The transplantation of MSCs alleviated liver fibrosis and reduced expression of transforming growth factor-β1, Smad2, collagen type Ⅰ, and α-SMA. HIR preconditioning promoted homing and repopulation of MSCs and resulted in better treatment outcomes. Conclusions HIR preconditioning enhances the effect of BM-MSCs in improving thioacetamide-induced liver fibrosis in rats by promoting their homing and repopulation. BM-MSCs may function by inhibiting transforming growth factor-β1-Smad signaling pathway in the liver. |
Databáze: | OpenAIRE |
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