Untargeted Metabolomics and Antioxidant Capacities of Muscadine Grape Genotypes during Berry Development
| Autor: | Islam El-Sharkawy, Mehboob B. Sheikh, Ahmed Gomaa Gomaa Darwish, Ahmed Ismail, Pranavkumar Gajjar, Protiva Rani Das, Violeta Tsolova, Sherif M. Sherif, Subramani Paranthaman Balasubramani |
|---|---|
| Přispěvatelé: | Alson H. Smith Jr. Agricultural Research and Extension Center, School of Plant and Environmental Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Antioxidant Physiology DPPH medicine.medical_treatment Clinical Biochemistry nutritional biomarkers RM1-950 Berry developmental stages Biology 01 natural sciences Biochemistry Article Veraison 03 medical and health sciences chemistry.chemical_compound Metabolome medicine Gallic acid Food science Molecular Biology 010401 analytical chemistry Catechin Cell Biology metabolomics 0104 chemical sciences 030104 developmental biology chemistry muscadine genotypes Therapeutics. Pharmacology Malic acid |
| Zdroj: | Antioxidants Volume 10 Issue 6 Antioxidants, Vol 10, Iss 914, p 914 (2021) |
| ISSN: | 2076-3921 |
| Popis: | Three muscadine grape genotypes (Muscadinia rotundifolia (Michx.) Small) were evaluated for their metabolite profiling and antioxidant activities at different berry developmental stages. A total of 329 metabolites were identified using UPLC-TOF-MS analysis (Ultimate 3000LC combined with Q Exactive MS and screened with ESI-MS) in muscadine genotypes throughout different developmental stages. Untargeted metabolomics study revealed the dominant chemical groups as amino acids, organic acids, sugars, and phenolics. Principal component analysis indicated that developmental stages rather than genotypes could explain the variations among the metabolic profiles of muscadine berries. For instance, catechin, epicatechin-3-gallate, and gallic acid were more accumulated in ripening seeds (RIP-S). However, tartaric acid and malonic acid were more abundant during the fruit-set (FS) stage, and malic acid was more abundant in the veraison (V) stage. The variable importance in the projection (VIP > 0.5) in partial least-squares–discriminant analysis described 27 biomarker compounds, representing the muscadine berry metabolome profiles. A heatmap of Pearson’s correlation analysis between the 27 biomarker compounds and antioxidant activities was able to identify nine antioxidant determinants among them, gallic acid, 4-acetamidobutanoic acid, trehalose, catechine, and epicatechin-3-gallate displayed the highest correlations with different types of antioxidant activities. For instance, DPPH and FRAP conferred a similar antioxidant activity pattern and were highly correlated with gallic acid and 4-acetamidobutanoic acid. This comprehensive study of the metabolomics and antioxidant activities of muscadine berries at different developmental stages is of great reference value for the plant, food, pharmaceutical, and nutraceutical sectors. |
| Databáze: | OpenAIRE |
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