| Autor: |
A. H. A. Damen, C. C. van Donkelaar, P. K. Sharma, H. Wan, R. Cardinaels, T. A. Schmidt, K. Ito |
| Přispěvatelé: |
Orthopaedic Biomechanics, Immunoengineering, ICMS Affiliated, Group Anderson, Processing and Performance, ICMS Core, Eindhoven MedTech Innovation Center |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Journal of Biomedical Materials Research, Part B: Applied Biomaterials, 111(8), 1523-1532. Wiley |
| ISSN: |
1552-4973 |
| Popis: |
Frictional properties of cartilage resurfacing implants should be sufficiently low to limit damaging of the opposing cartilage during articulation. The present study determines if native lubricious molecule proteoglycan 4 (PRG4) can adsorb onto a layer-by-layer bioinspired coating composed of poly-l-lysine (PLL) and dopamine modified hyaluronic acid (HADN) and thereby can reduce the friction between implant and articular cartilage. An ELISA was developed to quantify the amount of immobilized human recombinant (rh)PRG4 after exposure to the PLL-HADN coating. The effect on lubrication was evaluated by comparing the coefficient of friction (CoF) of bare polycaprolactone (PCL) disks to that of PLL-HADN coated PCL disks while articulated against cartilage using a ring-on-disk geometry and a lubricant solution consisting of native synovial fluid components including rhPRG4. The PLL-HADN coating effectively immobilized rhPRG4. The surface roughness of PCL disks significantly increased while the water contact angle significantly decreased after application of the coating. The average CoF measured during the first minute of bare PCL against cartilage exceeded twice the CoF of the PLL-HADN coated PCL against cartilage. After 60 min, the CoF reached equilibrium values which were still significantly higher for bare PCL compared to coated PCL. The present study demonstrated that PCL can effectively be coated with PLL-HADN. Additionally, this coating reduces the friction between PCL and cartilage when a PRG4-rich lubricant is used, similar to the lubricating surface of native cartilage. This makes PLL-HADN coating a promising application to improve the clinical success of PCL-based cartilage resurfacing implants. ispartof: Journal Of Biomedical Materials Research Part B-Applied Biomaterials vol:111 issue:8 ispartof: location:United States status: published |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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