EXPANSION OF THE INNER MEMBRANE COMPARTMENT AND ITS RELATION TO MITOCHONDRIAL VOLUME AND ION TRANSPORT
| Autor: | Lester Packer, J. M. Wrigglesworth, B. C Pressman, P. A. G. Fortes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1968 |
| Předmět: |
Osmosis
Light Biological Transport Active Mitochondria Liver Biology Article Ion Valinomycin chemistry.chemical_compound Methods Inner membrane Animals Compartment (pharmacokinetics) skin and connective tissue diseases Ion transporter Aldehydes Membranes Cell Biology Anti-Bacterial Agents Rats Microscopy Electron Membrane Biochemistry Volume (thermodynamics) chemistry Biophysics Potassium sense organs |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Glutaraldehyde has been used to fix mitochondria undergoing rapid volume changes associated with energized ion transport under oscillatory state conditions and valinomycin-induced potassium uptake. Fixation was found to prevent structural changes which normally occur during ion accumulation or loss. By correlating packed volume measurements with electron microscopy, it is shown that changes in volume associated with ion movements reflect changes in the inner membrane compartment and that this compartment can be related to the sucrose inaccessible space. The method can therefore be used to accurately determine volume changes that arise from ion translocation. |
| Databáze: | OpenAIRE |
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