Genome-scale metabolic network of Cordyceps militaris useful for comparative analysis of entomopathogenic fungi
| Autor: | Wanwipa Vongsangnak, Nam Ninh Nguyen, Nachon Raethong, Hon Wai Leong, Kobkul Laoteng, Warasinee Mujchariyakul |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Hydrolases Metabolic network Fungal Proteins 03 medical and health sciences chemistry.chemical_compound Biosynthesis Cordyceps militaris Ribonucleotide Reductases Genetics Gene chemistry.chemical_classification 030102 biochemistry & molecular biology biology Cordycepin Deoxyadenosines Virulence General Medicine Peroxisome biology.organism_classification Metabolic pathway 030104 developmental biology Enzyme chemistry Biochemistry Cordyceps Genome Fungal Metabolic Networks and Pathways |
| Zdroj: | Gene. 626 |
| ISSN: | 1879-0038 |
| Popis: | The first genome-scale metabolic network of Cordyceps militaris (iWV1170) was constructed representing its whole metabolisms, which consisted of 894 metabolites and 1,267 metabolic reactions across five compartments, including the plasma membrane, cytoplasm, mitochondria, peroxisome and extracellular space. The iWV1170 could be exploited to explain its phenotypes of growth ability, cordycepin and other metabolites production on various substrates. A high number of genes encoding extracellular enzymes for degradation of complex carbohydrates, lipids and proteins were existed in C. militaris genome. By comparative genome-scale analysis, the adenine metabolic pathway towards putative cordycepin biosynthesis was reconstructed, indicating their evolutionary relationships across eleven species of entomopathogenic fungi. The overall metabolic routes involved in the putative cordycepin biosynthesis were also identified in C. militaris, including central carbon metabolism, amino acid metabolism (glycine, l -glutamine and l -aspartate) and nucleotide metabolism (adenosine and adenine). Interestingly, a lack of the sequence coding for ribonucleotide reductase inhibitor was observed in C. militaris that might contribute to its over-production of cordycepin. |
| Databáze: | OpenAIRE |
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