Chrysophanol Suppresses Hypoxia-Induced Epithelial-Mesenchymal Transition in Colorectal Cancer Cells
| Autor: | Xiquan Ke, Xiaodong Chen, Jun Wei, Le-Rong Xu, Yongju Xue, Min Deng, Jianchao Wang, Qiangwu Wang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Histology Epithelial-Mesenchymal Transition Colorectal cancer Mice Nude Anthraquinones Apoptosis Metastasis Small hairpin RNA 03 medical and health sciences Mice 0302 clinical medicine Gentamicin protection assay Cell Movement medicine Tumor Cells Cultured Animals Humans Epithelial–mesenchymal transition Hypoxia Ecology Evolution Behavior and Systematics PI3K/AKT/mTOR pathway Cell Proliferation Mice Inbred BALB C Cell growth Chemistry Akt/PKB signaling pathway medicine.disease Xenograft Model Antitumor Assays 030104 developmental biology Cancer research Anatomy Colorectal Neoplasms 030217 neurology & neurosurgery Biotechnology Mutagens |
| Zdroj: | Anatomical record (Hoboken, N.J. : 2007). 302(9) |
| ISSN: | 1932-8494 |
| Popis: | Colorectal cancer (CRC) is a common human malignancy, accounting for 600,000 death cases annually worldwide. Chrysophanol is a naturally occurring anthraquinone compound and exhibits anti-neoplastic activities. This study aims to explore the biological effects of chrysophanol on CRC metastasis and the relevant underlying mechanism. Cell proliferation assay, wound scratch assay, and Transwell invasion assay were used to examine the effect of chrysophanol on proliferation, migration, and invasion of CRC cells. Hypoxia-inducible factor-1α (HIF-1α) shRNA was utilized to transfect CRC cells to examine the role of HIF-1α in chrysophanol suppression of hypoxia-induced epithelial to mesenchymal transition (EMT). The suppression effect of chrysophanol on hypoxia-induced EMT in vivo was also validated in xenograft tumor models. In the present study, our findings indicated that chrysophanol has the capability to suppress hypoxia-induced EMT in CRC in vitro and in vivo, and the possible mechanism involved is the inhibition of HIF-1α via modulating PI3k/Akt signaling pathway. Collectively, the results indicated that chrysophanol can be used as an EMT and cancer metastasis inhibitor in the treatment of CRC. Anat Rec, 302:1561-1570, 2019. © 2019 American Association for Anatomy. |
| Databáze: | OpenAIRE |
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