Bacterial community composition and diversity of two different forms of an organic residue of bioenergy crop
| Autor: | Matheus Aparecido Pereira Cipriano, Janaina Braga do Carmo, Afnan Khalil Ahmad Suleiman, Eiko E. Kuramae, Adriana Parada Dias da Silveira |
|---|---|
| Přispěvatelé: | Microbial Ecology (ME) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Environmental Impacts
Bacilli Denitrification Vinasse Denitrification pathway lcsh:Medicine Biology 7. Clean energy Microbiology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Bioenergy 16S rRNA Agricultural Science Nitrogen cycle 030304 developmental biology 2. Zero hunger 0303 health sciences Diversity Bacteria Biochemical characterization General Neuroscience lcsh:R 04 agricultural and veterinary sciences General Medicine Biodiversity 15. Life on land Sugarcane biology.organism_classification Microbial population biology Agronomy international Bacteria isolation 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Nitrification Nitrogen cycle genes General Agricultural and Biological Sciences |
| Zdroj: | PeerJ PeerJ, 7:e6768. PeerJ PeerJ, Vol 7, p e6768 (2019) |
| ISSN: | 2167-8359 |
| Popis: | The use of residue of sugarcane ethanol industry named vinasse in fertirrigation is an established and widespread practice in Brazil. Both non-concentrated vinasse (NCV) and concentrated vinasse (CV) are used in fertirrigation, particularly to replace the potassium fertilizer. Although studies on the chemical and organic composition of vinasse and their impact on nitrous oxide emissions when applied in soil have been carried out, no studies have evaluated the microbial community composition and diversity in different forms of vinasse. We assessed the bacterial community composition of NCV and CV by non-culturable and culturable approaches. The non-culturable bacterial community was assessed by next generation sequencing of the 16S rRNA gene and culturable community by isolation of bacterial strains and molecular and biochemical characterization. Additionally, we assessed in the bacterial strains the presence of genes of nitrogen cycle nitrification and denitrification pathways. The microbial community based on16S rRNAsequences of NCV was overrepresented by Bacilli and Negativicutes while CV was mainly represented by Bacilli class. The isolated strains from the two types of vinasse belong to class Bacilli, similar toLysinibacillus, encode fornirKgene related to denitrification pathway. This study highlights the bacterial microbial composition particularly in CV what residue is currently recycled and recommended as a sustainable practice in sugarcane cultivation in the tropics. |
| Databáze: | OpenAIRE |
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