Dopamine-dependent iron toxicity in cells derived from rat hypothalamus
| Autor: | Patricio Fuentes, Juan Segura-Aguilar, Claudio Olea-Azar, Sergio Cardenas, Pedro Martinez-Alvarado, Rebecca Graumann, Pablo Caviedes, Carolina Perez-Pastene, Irmgard Paris |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Dicumarol
Programmed cell death Dopamine Iron Morpholines Hypothalamus Fluorescent Antibody Technique Dopamine beta-Hydroxylase Toxicology Ferric Compounds Cell Line Reboxetine Chlorides NAD(P)H Dehydrogenase (Quinone) medicine Animals Indolequinones Microscopy Confocal Norepinephrine Plasma Membrane Transport Proteins Adrenergic Uptake Inhibitors Cell Death Symporters biology Chemistry Neurotoxicity General Medicine Dicoumarol medicine.disease Molecular biology Rats Inbred F344 Rats Biochemistry Norepinephrine transporter Cell culture One-electron reduction biology.protein Intracellular medicine.drug |
| Zdroj: | CHEMICAL RESEARCH IN TOXICOLOGY Artículos CONICYT CONICYT Chile instacron:CONICYT |
| Popis: | We report a new and specific mechanism for iron-mediated neurotoxicity using RCHT cells, which were derived from rat hypothalamus. RCHT cells exhibit immunofluorescent-positive markers for dopamine beta-hydroxylase and the norepinephrine transporter, NET. In the present study, we observed that iron-induced neurotoxicity in RCHT cells was dependent on (i) formation of an Fe-dopamine complex (100 microM FeCl3:100 microM dopamine); (ii) specific uptake of the Fe-dopamine complex into RCHT cells via NET (79+/-2 pmol 59Fe/mg/min; P |
| Databáze: | OpenAIRE |
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