Haplotypes of the HLA-G 3’ untranslated region respond to endogenous factors of HLA-G+ and HLA-G- cell lines differentially
| Autor: | Philippe Moreau, Isabelle Poras, Yara Costa Netto Muniz, Bibiana Sgorla de Almeida, Celso T. Mendes-Junior, Gustavo Martelli-Palomino, Natalia F. Cagnin, Eduardo Antônio Donadi, Layale Yaghi, Edgardo D. Carosella, Erick C. Castelli |
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| Přispěvatelé: | Hôpital Saint-Louis, School of Medicine, Universidade Federal do Rio Grande do Norte, Universidade de São Paulo (USP), Universidade Federal de Santa Catarina (UFSC), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Untranslated region Heredity lcsh:Medicine Biochemistry Database and Informatics Methods 0302 clinical medicine Post-Transcriptional Gene Regulation INDEL Mutation Genes Reporter HLA-G lcsh:Science Luciferases 3' Untranslated Regions Base Pairing Genetics Regulation of gene expression Multidisciplinary 3. Good health Enzymes Gene Expression Regulation Neoplastic Nucleic acids Genetic Mapping Oxidoreductases Luciferase Sequence Analysis Research Article Bioinformatics Human leukocyte antigen Biology Research and Analysis Methods POLIMORFISMO 03 medical and health sciences Gene Types Sequence Motif Analysis Cell Line Tumor Genetic predisposition Humans Non-coding RNA Gene DNA sequence analysis HLA-G Antigens Polymorphism Genetic Base Sequence Three prime untranslated region lcsh:R Haplotype Biology and Life Sciences Proteins Molecular biology Gene regulation MicroRNAs 030104 developmental biology Haplotypes Mutagenesis Site-Directed Enzymology RNA lcsh:Q Gene expression 030215 immunology Reporter Genes |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP PLoS ONE Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP PLoS ONE, Vol 12, Iss 1, p e0169032 (2017) |
| Popis: | Made available in DSpace on 2018-12-11T17:23:20Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The immune checkpoint HLA-G prevents maternal rejection of the fetus and contributes in cancer invasion and acceptance of allografts. The 5′ and 3′ regulatory regions of the HLA-G gene are polymorphic and balancing selection probably maintains this variability. It is proposed that nucleotide variations may affect the level of HLA-G expression. To investigate this issue we aimed to analyze how haplotypes of the 3′ untranslated region (3′UTR) with highest worldwide frequencies, namely UTR-1, UTR-2, UTR-3, UTR-4, UTR-5, UTR-18 and UTR-7, impact the expression of a luciferase reporter gene in vitro. Experiments performed with the HLA-G positive cell lines JEG-3 (choricarcinoma) and FON (melanoma), and with the HLA-G negative cell lines M8 (melanoma) and U251MG (glioblastoma) showed that the HLA-G 3′UTR polymorphism influences the response to endogenous cellular factors and may vary according to the cell type. UTR-5 and UTR-7 impact the activity of luciferase the most whereas UTR-2, UTR-3, UTR-4, and UTR-18 have intermediate impact, and UTR-1 has the lowest impact. These results corroborate the previous associations between amounts of plasma sHLA-G levels and 3′UTR haplotypes in healthy individuals and reinforce that 3′UTR typing may be a predictor of the genetic predisposition of an individual to express different levels of HLA-G. Commissariat à l'Energie Atomique et aux Energies Alternatives Institut des Maladies Emergentes et des Thérapies Innovantes Service de Recherches en Hémato-Immunologie Hôpital Saint-Louis Université Paris-Diderot Sorbonne Paris-Cité UMR-E5 Institut Universitaire D'Hématologie Hôpital Saint-Louis Lebanese University School of Medicine Programa de Pósgraduação em Ciências da Saúde Centro de Ciências da Saúde Universidade Federal do Rio Grande do Norte Departamento de Química Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo Departamento de Biologia Celular Embriologia e Genética Centro de Ciências Biológicas Universidade Federal de Santa Catarina Department of Genetics Ribeirão Preto Medical School University of São Paulo Divisão de Imunologia Clínica Departamento de Clínica Médica Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo Department of Pathology School of Medicine Universidade Estadual Paulista Unesp Department of Pathology School of Medicine Universidade Estadual Paulista Unesp CAPES: 014747/2013-8 FAPESP: 2014/18730-9 CNPq: 406594/2013-9 |
| Databáze: | OpenAIRE |
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