Resveratrol reverses morphine-induced neuroinflammation in morphine-tolerant rats by reversal HDAC1 expression
| Autor: | Juei-Chii Wang, Ru-Yin Tsai, Chih-Shung Wong, Kuang-Yi Chou, Chen-Hwan Cherng |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
intrathecal
Male medicine.medical_treatment spinal Histone Deacetylase 1 Pharmacology Resveratrol 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine 030202 anesthesiology Stilbenes medicine Animals Receptors Tumor Necrosis Factor Type II Rats Wistar Neuroinflammation Injections Spinal Medicine(all) lcsh:R5-920 Morphine business.industry Tumor Necrosis Factor-alpha Anti-Inflammatory Agents Non-Steroidal General Medicine Drug Tolerance Rats Tolerance induction Nociception Cytokine Opioid chemistry Spinal Cord Receptors Tumor Necrosis Factor Type I Anesthesia Neuropathic pain opioid Cytokines Neuralgia business lcsh:Medicine (General) 030217 neurology & neurosurgery epigenetic medicine.drug |
| Zdroj: | Journal of the Formosan Medical Association, Vol 115, Iss 6, Pp 445-454 (2016) |
| ISSN: | 0929-6646 |
| DOI: | 10.1016/j.jfma.2015.05.010 |
| Popis: | Background/purpose We previously showed that subsequent intrathecal (i.t.) injection of resveratrol (30 μg) significantly reverses morphine-evoked neuroinflammation in morphine-tolerant rats. The present study examined the underlying mechanism. Methods Male Wistar rats were implanted with two i.t. catheters, one of which was connected to a miniosmotic pump and used for morphine (15 μg/h) or saline infusion for 120 hours. To examine the effects on spinal cord expression of histone deacetylase 1 (HDAC1), the inflammatory cytokine tumor necrosis factor-α (TNF-α), and TNF receptor (TNFR) 1 and TNFR2 during tolerance induction, a tail-flick test was performed prior to infusion and after 24 hours, 48 hours, 72 hours, 96 hours, and 120 hours of infusion. Results Resveratrol treatment prior to morphine challenge restored the antinociceptive effect of morphine in morphine-tolerant rats and reversed the morphine infusion-induced increase in HDAC1, TNF-α, and TNFR1 expression. Moreover, chronic morphine infusion increased TNFR1-specific expression in neuron in morphine-tolerant rat spinal cords, and this effect was almost completely inhibited by resveratrol treatment prior to morphine challenge. Conclusion Resveratrol restores the antinociceptive effect of morphine by reversing morphine infusion-induced spinal cord neuroinflammation and increase in TNFR1 expression. The reversal of the morphine-induced increase in TNFR1 expression by resveratrol is partially due to reversal of the morphine infusion-induced increase in HDAC1 expression. Resveratrol pretreatment can be used as an adjuvant in clinical pain management for patients who need long-term morphine treatment or with neuropathic pain. |
| Databáze: | OpenAIRE |
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