Sac7 and Rho1 regulate the white-to-opaque switching in Candida albicans
| Autor: | Guisheng Zeng, Yan-Ming Wang, Dongliang Yang, Siwy Ling Yang, Fong Yee Chan, Yue Wang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
rho GTP-Binding Proteins
0301 basic medicine medicine.medical_specialty 030106 microbiology Mutant lcsh:Medicine Locus (genetics) Haploidy Biology Article 03 medical and health sciences Gene Expression Regulation Fungal Molecular genetics Candida albicans medicine Amino Acid Sequence lcsh:Science Transcription factor Multidisciplinary lcsh:R GTPase-Activating Proteins Candidiasis biology.organism_classification Diploidy Corpus albicans Cell biology Phenotype 030104 developmental biology Mutation cAMP-dependent pathway lcsh:Q Signal transduction Protein Binding |
| Zdroj: | Scientific Reports Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-19246-9 |
| Popis: | Candida albicans cells homozygous at the mating-type locus stochastically undergo the white-to-opaque switching to become mating-competent. This switching is regulated by a core circuit of transcription factors organized through interlocking feedback loops around the master regulator Wor1. Although a range of distinct environmental cues is known to induce the switching, the pathways linking the external stimuli to the central control mechanism remains largely unknown. By screening a C. albicans haploid gene-deletion library, we found that SAC7 encoding a GTPase-activating protein of Rho1 is required for the white-to-opaque switching. We demonstrate that Sac7 physically associates with Rho1-GTP and the constitutively active Rho1G18V mutant impairs the white-to-opaque switching while the inactive Rho1D124A mutant promotes it. Overexpressing WOR1 in both sac7Δ/Δ and rho1 G18V cells suppresses the switching defect, indicating that the Sac7/Rho1 module acts upstream of Wor1. Furthermore, we provide evidence that Sac7/Rho1 functions in a pathway independent of the Ras/cAMP pathway which has previously been positioned upstream of Wor1. Taken together, we have discovered new regulators and a signaling pathway that regulate the white-to-opaque switching in the most prevalent human fungal pathogen C. albicans. |
| Databáze: | OpenAIRE |
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