Ganglioside synthesis by plasma membrane-associated sialyltransferase in macrophages
| Autor: | Vanina Torres Demichelis, Eduardo Garbarino-Pico, Jose L. Daniotti, Aldo Alejandro Vilcaes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Lipopolysaccharides
0301 basic medicine GANGLIOSIDES sialyltransferase Lipopolysaccharide Cell Endogeny GLYCOSPHINGOLIPIDS lcsh:Chemistry purl.org/becyt/ford/1 [https] Mice chemistry.chemical_compound 0302 clinical medicine Macrophage lcsh:QH301-705.5 Spectroscopy CIS-CATALYTIC ACTIVITY glycosphingolipids biology General Medicine gangliosides Computer Science Applications Cell biology medicine.anatomical_structure 030220 oncology & carcinogenesis lipids (amino acids peptides and proteins) NITRIC OXIDE SIALYLTRANSFERASE Sialyltransferase CHO Cells G(M1) Ganglioside macrophage Article Catalysis Cell Line Nitric oxide Inorganic Chemistry 03 medical and health sciences Cricetulus Immune system nitric oxide medicine Animals Physical and Theoretical Chemistry purl.org/becyt/ford/1.6 [https] Molecular Biology Ganglioside Lipogenesis Macrophages Cell Membrane Organic Chemistry Sialyltransferases RAW 264.7 Cells 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 chemistry biology.protein MACROPHAGE cis-catalytic activity |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET International Journal of Molecular Sciences, Vol 21, Iss 3, p 1063 (2020) International Journal of Molecular Sciences Volume 21 Issue 3 |
| Popis: | Gangliosides are constituents of the mammalian cell membranes and participate in the inflammatory response. However, little is known about the presence and enzymatic activity of ganglioside sialyltransferases at the cell surface of macrophages, one of the most important immune cells involved in the innate inflammatory process. In the present study, using biochemical and fluorescent microscopy approaches, we found that endogenous ST8Sia-I is present at the plasma membrane (ecto-ST8Sia-I) of murine macrophage RAW264.7 cells. Moreover, ecto-ST8Sia-I can synthetize GD3 ganglioside at the cell surface in lipopolysaccharide (LPS)-stimulated macrophages even when LPS-stimulated macrophages reduced the total ST8Sia-I expression levels. Besides, cotreatment of LPS with an inhibitor of nitric oxide (NO) synthase recovered the ecto-ST8Sia-I expression, suggesting that NO production is involved in the reduction of ST8Sia-I expression. The diminution of ST8Sia-I expression in LPS-stimulated macrophages correlated with a reduction of GD3 and GM1 gangliosides and with an increment of GD1a. Taken together, the data supports the presence and activity of sialyltransferases at the plasma membrane of RAW264.7 cells. The variations of ecto-ST8Sia-I and ganglioside levels in stimulated macrophages constitutes a promissory pathway to further explore the physiological role of this and others ganglioside metabolism-related enzymes at the cell surface during the immune response. Fil: Vilcaes, Aldo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina Fil: Garbarino Pico, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina Fil: Torres Demichelis, Vanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina Fil: Daniotti, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Química Biológica; Argentina |
| Databáze: | OpenAIRE |
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