Gas chromatographic fingerprint analysis of secondary metabolites of Stachys lanata (Stachys byzantine C. Koch) combined with antioxidant activity modelling using multivariate chemometric methods
| Autor: | Hadi Parastar, Mahtab Abtahi, Parimah Aminfar |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Multivariate statistics
Chromatography Gas Antioxidant medicine.medical_treatment Secondary Metabolism 010402 general chemistry 01 natural sciences Biochemistry Antioxidants Analytical Chemistry Chemometrics Partial least squares regression medicine Least-Squares Analysis Principal Component Analysis Chromatography biology Chemistry Vitamin E 010401 analytical chemistry Organic Chemistry Discriminant Analysis General Medicine Stachys biology.organism_classification 0104 chemical sciences Multivariate Analysis Chromatographic fingerprint Gas chromatography |
| Zdroj: | Journal of Chromatography A. 1602:432-440 |
| ISSN: | 0021-9673 |
| Popis: | S. lanata has been traditionally used as a medicinal plant due to its various biological activities such as antioxidant activity. Therefore, identification and quality control studies of this plant are of great importance. To this end, gas chromatography (GC) combined with chemometrics was proposed for fingerprint analysis of S. lanata samples. This study sought to classify GC fingerprints of twenty-eight S. lanata samples from eight different regions of Iran and more importantly, to correlate fingerprints to the antioxidant activity to select S. lanata volatile antioxidant markers. S. lanata samples were classified into five and three classes using partial least squares-discriminant analysis (PLS-DA) according to their GC fingerprints and antioxidant peaks, respectively. The results of PLS regression (PLS-R) and variable importance in projection (VIP) showed that phenol, 2,4-bis (1,1-dimethylethyl)-, hexadecanoic acid- ethyl ester, vitamin E, Beta- sitosterol, and 1- monolinoleoylglycerol trimethylsily ether are volatile antioxidant markers of S. lanata samples. |
| Databáze: | OpenAIRE |
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