Association of expression of inflammatory response genes and DNA repair genes in colorectal carcinoma
| Autor: | Beatriz da Costa Aguiar Alves Reis, Leonardo Cardili, Fernando Luiz Affonso Fonseca, Flávia de Sousa Gehrke, Thérèse Rachell Theodoro, Maria Isete Fares Franco, Demétrius Eduardo Germini, Jaques Waisberg, Celina Tizuko Fujiyama Oshima |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine congenital hereditary and neonatal diseases and abnormalities DNA Repair Carcinogenesis DNA repair Colorectal cancer Inflammatory response Inflammation 03 medical and health sciences 0302 clinical medicine medicine Humans Gene RC254-282 Aged Cell Proliferation Mismatch Repair Endonuclease PMS2 Aged 80 and over Interleukin-6 Tumor Necrosis Factor-alpha business.industry Interleukin Neoplasms. Tumors. Oncology. Including cancer and carcinogens General Medicine Middle Aged medicine.disease digestive system diseases Interleukin-10 DNA-Binding Proteins Gene Expression Regulation Neoplastic MutS Homolog 2 Protein 030104 developmental biology 030220 oncology & carcinogenesis Disease Progression Cancer research Interleukin-2 Female DNA mismatch repair Tumor necrosis factor alpha medicine.symptom Colorectal Neoplasms MutL Protein Homolog 1 business |
| Zdroj: | Tumor Biology, Vol 41 (2019) |
| ISSN: | 1423-0380 |
| Popis: | Inflammation is an important etiological factor of colorectal carcinoma and may be related to colorectal carcinoma growth and proliferation. This study aimed to verify whether the presence of chronic inflammation represented by tumor necrosis factor-α, interleukin-2, interleukin-6, and interleukin-10 gene expression is related to hMLH1, hMSH2, hMSH6, and PMS2 gene expression and the corresponding protein levels of these genes from the DNA repair system. A total of 83 patients were operated on for curative or palliative colorectal carcinoma. Expression of the inflammatory response genes tumor necrosis factor-α, interleukin-2, interleukin-6, and interleukin-10 as well as expression of the hMLH1, hMSH2, hMSH6, and PMS2 genes of the DNA repair system (mismatch repair) and the expression levels of the corresponding mismatch repair proteins were measured in neoplastic tissue by reverse transcription polymerase chain reaction and immunohistochemistry, respectively. Associations were observed between hMSH6 mRNA expression and interleukin-2 mRNA expression (p = 0.026) as well as between hMLH1 and hMSH2 gene expression and tumor necrosis factor-α gene expression (p = 0.042). Higher tissue levels of interleukin-2 and tumor necrosis factor-α gene expression were associated with lower hMSH6, hMLH1, and hMSH2 gene expression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|