Expression of ribosomal and translation-associated genes is correlated with a favorable clinical course in chronic lymphocytic leukemia
Autor: | Fabio Malavasi, Anja Führer, Holger Nückel, Jan Dürig, Tarik Möröy, Naser Kalhori, Silvia Deaglio, Roland Rudolph, Tanja Hölter, Ulrich Dührsen, Elisabeth Kruse, Andreas Hüttmann, Arnd Nusch, Katja Halfmeyer, Ludger Klein-Hitpass |
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Rok vydání: | 2003 |
Předmět: |
Male
Ribosomal Proteins Chronic lymphocytic leukemia Immunology Medizin Clone (cell biology) Biology CD38 Biochemistry Antigens CD Risk Factors immune system diseases hemic and lymphatic diseases Gene expression medicine Cluster Analysis Humans Translation factor ADP-ribosyl Cyclase Gene Aged Oligonucleotide Array Sequence Analysis Membrane Glycoproteins Gene Expression Profiling Cell Biology Hematology Middle Aged Prognosis medicine.disease ADP-ribosyl Cyclase 1 Leukemia Lymphocytic Chronic B-Cell Gene expression profiling Phenotype Treatment Outcome Protein Biosynthesis Cancer research Female DNA microarray |
Zdroj: | Blood. 101:2748-2755 |
ISSN: | 1528-0020 0006-4971 |
Popis: | B-cell chronic lymphocytic leukemia (B-CLL) is a heterogenous disease with a highly variable clinical course. Recent studies have shown that CD38 surface expression on the malignant cell clone may serve as a prognostic marker in that CD38+ patients with B-CLL are characterized by advanced disease stage, lesser responsiveness to chemotherapy, and shorter survival than CD38− patients. To further investigate the molecular phenotype of these 2 clinical subgroups, we compared the gene expression profiles of CD38+ (n = 25) with CD38− (n = 45) B-CLL patients using oligonucleotide-based DNA chip microarrays representative of approximately 5600 genes. The results showed that B-CLLs display a common gene expression profile that is largely independent of CD38 expression. Nonetheless, the expression of 14 genes differed significantly between the 2 groups, including genes that are involved in the regulation of cell survival. Furthermore, unsupervised hierarchical cluster analysis of 76 B-CLL samples led to the separation of 2 major subgroups, comprising 20 and 56 patients. Clustering to the smaller group was due in part to the coordinate high expression of a large number of ribosomal and other translation-associated genes, including elongation factors. Importantly, we found that patients with high expression of translation factors were characterized by a more favorable clinical course with significantly longer progression-free survival and reduced chemotherapy requirements than the remaining patients (P |
Databáze: | OpenAIRE |
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