Diabetic encephalopathy: beneficial effects of supplementation with fatty acids ω3 and nordihydroguaiaretic acid in a spontaneous diabetes rat model

Autor: M.E. Pasqualini, Aldo R. Eynard, Cristina B. López, Gastón Repossi, Alejandro Dain, Gustavo Tomás Díaz-Gerevini
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Blood Glucose
Male
Antioxidant
Endocrinology
Diabetes and Metabolism
medicine.medical_treatment
Clinical Biochemistry
Type 2 diabetes
030204 cardiovascular system & hematology
medicine.disease_cause
Hippocampus
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Polyunsaturated fatty acids ω3
Nordihydroguaiaretic acid
Diabetic Neuropathies
Medicine
lcsh:RC620-627
chemistry.chemical_classification
Brain Diseases
Brain
lcsh:Nutritional diseases. Deficiency diseases
eSS rats
diabetes mellitus
Polyunsaturated fatty acid
Lipidology
medicine.medical_specialty
Clinical chemistry
030209 endocrinology & metabolism
03 medical and health sciences
Diabetic encephalopathy
Diabetes mellitus
Internal medicine
Fatty Acids
Omega-3
Animals
Masoprocol
Rats
Wistar
business.industry
Research
Biochemistry (medical)
Glucose Tolerance Test
medicine.disease
Rats
Disease Models
Animal
Oxidative Stress
chemistry
Dietary Supplements
business
Oxidative stress
Zdroj: Lipids in Health and Disease, Vol 18, Iss 1, Pp 1-15 (2019)
Lipids in Health and Disease
DOI: 10.1186/s12944-018-0938-7
Popis: Background Diabetic encephalopathy is a chronic complications of diabetes mellitus that affects the central nervous system. We evaluated the effect of ω3 and ω6 polyunsaturated fatty acids (PUFAs) supplementation plus the antioxidant agent nordihydroguaiaretic acid (NDGA) on the etiopathology of diabetic encephalopathy in eSS rats, a spontaneous model of type 2 diabetes. Methods One hundred twenty spontaneous diabetic eSS male rats and 38 non-diabetic Wistar, used as healthy control, received monthly by intraperitoneal route, ω3 or ω6 PUFA (6.25 mg/kg) alone or plus NDGA (1.19 mg/kg) for 12 months. Diabetic rats had a worse performance in behavioural Hole-Board test. Histopathological analysis confirmed lesions in diabetic rats brain tissues. We also detected low expression of synaptophysin, a protein linked to release of neurotransmitters, by immunohistochemically techniques in eSS rats brain. Biochemical and histopathological studies of brain were performed at 12th month. Biochemical analysis showed altered parameters related to metabolism. High levels of markers of oxidative stress and inflammation were detected in plasma and brain tissues. Data were analysed by ANOVA test and paired t test was used by comparison of measurements of the same parameter at different times. Results The data obtained in this work showed that behavioural, biochemical and morphological alterations observed in eSS rats are compatible with previously reported indices in diabetic encephalopathy and are associated with increased glucolipotoxicity, chronic low-grade inflammation and oxidative stress burden. Experimental treatments assayed modulated the values of studied parameters. Conclusions The treatments tested with ω3 or ω3 plus NDGA showed improvement in the values of the studied parameters in eSS diabetic rats. These observations may form the basis to help in prevent and manage the diabetic encephalopathy. Electronic supplementary material The online version of this article (10.1186/s12944-018-0938-7) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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