A Novel Glucose Sensor Using Lutetium Phthalocyanine as Redox Mediator in Reduced Graphene Oxide Conducting Polymer Multifunctional Hydrogel

Autor: H. Al-Sagur, Ayşe G. Gürek, Khan, Shanmugasundaram Komathi, Aseel Hassan
Rok vydání: 2017
Předmět:
Blood Glucose
0301 basic medicine
Indoles
02 engineering and technology
Biosensing Techniques
Isoindoles
Lutetium
01 natural sciences
Hydrogel
Polyethylene Glycol Dimethacrylate
law.invention
chemistry.chemical_compound
Limit of Detection
law
Polyaniline
Electrochemistry
Organic chemistry
Glucose oxidase
General Environmental Science
Conductive polymer
Aniline Compounds
biology
Polyacrylic acid
Oxides
General Medicine
021001 nanoscience & nanotechnology
Graphite
0210 nano-technology
Oxidation-Reduction
Biotechnology
Materials science
Radical polymerization
Biomedical Engineering
Biophysics
010402 general chemistry
Glucose Oxidase
03 medical and health sciences
Humans
Detection limit
030109 nutrition & dietetics
Graphene
010401 analytical chemistry
Electrochemical Techniques
Enzymes
Immobilized
0104 chemical sciences
Chemical engineering
chemistry
Phthalocyanine
biology.protein
General Earth and Planetary Sciences
Biosensor
Nuclear chemistry
Zdroj: Procedia Technology. 27:183-184
ISSN: 2212-0173
1873-4235
DOI: 10.1016/j.protcy.2017.04.078
Popis: Herein, we report a scalable synthesis of multifunctional conducting polyacrylic acid (PAA) hydrogel (MFH) integrated with reduced grapheme oxide (rGO), vinyl substituted polyaniline (VS-PANI) and lutetium Phthalocyanine (LuPc2) as three dimensional robust matrix for glucose oxidase (GOx) immobilization (PAA-rGO/VS-PANI/LuPc2/GOx-MFH). We have integrated the multicomponents such as PAA with rGO, and VS-PANI through free radical polymerization using methylene bis-acrylamide, and ammonium persulphate as the cross linker and initiator. The LuPc2 was then doped to form multifunctional hydrogel (PAA-rGO/VS-PANI/LuPc2-MFH). Finally, biosensor was fabricated by immobilizing GOx into PAA-rGO/VS-PANI/LuPc2-MFH and subsequently used for electrochemical detection of glucose. The PAA-rGO/VS-PANI/LuPc2/GOx-MFH biosensor exhibited high sensitivity (15.31 μA mM−1 cm−2) for the detection of glucose over a concentration range of 2–12 mM with a low detection limit of 25 µm. The PAA-rGO/VS-PANI/LuPc2-MFH biosensor showed a fast response time (1 s) to the addition of glucose with high storage stability of 3 months. The real sample analysis reveals that PAA-rGO/VS-PANI/LuPc2/GOx-MFH could be effectively used as an electrochemical biosensor in industrial as well clinical diagnosis.
Databáze: OpenAIRE
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