Activation of the mismatch-specific endonuclease EndoMS/NucS by the replication clamp is required for high fidelity DNA replication
| Autor: | Yoshizumi Ishino, Hanae Kudo, Asmae Es-Sadik, Caroline L'Hermitte-Stead, Sonoko Ishino, Jean Christophe Lambry, Stéphane Skouloubris, Hannu Myllykallio |
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| Přispěvatelé: | Department of Bioscience and Biotechnology [Fukuoka], Graduate School of Bioresource and Bioenvironmental Sciences [Fukuoka], Kyushu University [Fukuoka]-Kyushu University [Fukuoka], Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Optique et Biosciences (LOB), École polytechnique (X)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École polytechnique (X) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
DNA Replication
0301 basic medicine Base Pair Mismatch [SDV]Life Sciences [q-bio] DNA-Directed DNA Polymerase medicine.disease_cause Corynebacterium glutamicum 03 medical and health sciences chemistry.chemical_compound Endonuclease MutL Proteins Bacterial Proteins Genetics medicine [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Molecular Biology Mutation [SDV.GEN]Life Sciences [q-bio]/Genetics Endodeoxyribonucleases 030102 biochemistry & molecular biology biology DNA replication DNA Actinobacteria 030104 developmental biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology chemistry biology.protein Replisome DNA mismatch repair |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2018, ⟨10.1093/nar/gky460⟩ |
| ISSN: | 0305-1048 1362-4962 |
| DOI: | 10.1093/nar/gky460⟩ |
| Popis: | International audience; The mismatch repair (MMR) system, exemplified by the MutS/MutL proteins, is widespread in Bacteria and Eukarya. However, molecular mechanisms how numerous archaea and bacteria lacking the mutS/mutL genes maintain high replication fidelity and genome stability have remained elusive. EndoMS is a recently discovered hyperthermophilic mismatch-specific endonuclease encoded by nucS in Thermococcales. We deleted the nucS from the actinobacterium Corynebacterium glutamicum and demonstrated a drastic increase of spontaneous transition mutations in the nucS deletion strain. The observed spectra of these mutations were consistent with the enzymatic properties of EndoMS in vitro. The robust mismatch-specific endonuclease activity was detected with the purified C. glutamicum EndoMS protein but only in the presence of the β-clamp (DnaN). Our biochemical and genetic data suggest that the frequently occurring G/T mismatch is efficiently repaired by the bacterial EndoMS-β−clamp complex formed via a carboxy-terminal sequence motif of EndoMS proteins. Our study thus has great implications for understanding how the activity of the novel MMR system is coordinated with the replisome and provides new mechanistic insight into genetic diversity and mutational patterns in industrially and clinically (e.g. Mycobacteria) important archaeal and bacterial phyla previously thought to be devoid of the MMR system. |
| Databáze: | OpenAIRE |
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