Detection and measurement of surface contamination by multiple antineoplastic drugs using multiplex bead assay
Autor: | D. Gayle DeBord, Shirley A. Robertson, Deborah L. Sammons, John E. Snawder, Jack R. Pretty, Thomas H. Connor, Jerome P. Smith |
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Rok vydání: | 2014 |
Předmět: |
Drug
Paclitaxel Drug Contamination media_common.quotation_subject Antineoplastic Agents Pharmacology Tandem mass spectrometry Mass spectrometry Article 03 medical and health sciences 0302 clinical medicine Limit of Detection Tandem Mass Spectrometry Occupational Exposure Humans Medicine Pharmacology (medical) Multiplex Workplace Immunosorbent Techniques media_common Detection limit Chromatography business.industry Microbead (research) Contamination 030210 environmental & occupational health Microspheres Oncology Doxorubicin 030220 oncology & carcinogenesis Fluorouracil business Chromatography Liquid |
Zdroj: | Journal of Oncology Pharmacy Practice. 22:60-67 |
ISSN: | 1477-092X 1078-1552 |
Popis: | Objectives Contamination of workplace surfaces by antineoplastic drugs presents an exposure risk for healthcare workers. Traditional instrumental methods to detect contamination such as liquid chromatography–mass spectrometry/mass spectrometry (LC–MS/MS) are sensitive and accurate but expensive. Since immunochemical methods may be cheaper and faster than instrumental methods, we wanted to explore their use for routine drug residue detection for preventing worker exposure. Methods In this study we examined the feasibility of using fluorescence covalent microbead immunosorbent assay (FCMIA) for simultaneous detection and semi-quantitative measurement of three antineoplastic drugs (5-fluorouracil, paclitaxel, and doxorubicin). The concentration ranges for the assay were 0–1000 ng/ml for 5-fluorouracil, 0–100 ng/ml for paclitaxel, and 0–2 ng/ml for doxorubicin. The surface sampling technique involved wiping a loaded surface with a swab wetted with wash buffer, extracting the swab in storage/blocking buffer, and measuring drugs in the extract using FCMIA. Results There was no significant cross-reactivity between these drugs at the ranges studied indicated by a lack of response in the assay to cross analytes. The limit of detection (LOD) for 5-fluorouracil on the surface studied was 0.93 ng/cm2 with a limit of quantitation (LOQ) of 2.8 ng/cm2, the LOD for paclitaxel was 0.57 ng/cm2 with an LOQ of 2.06 ng/cm2, and the LOD for doxorubicin was 0.0036 ng/cm2 with an LOQ of 0.013 ng/cm2. Conclusion The use of FCMIA with a simple sampling technique has potential for low cost simultaneous detection and semi-quantitative measurement of surface contamination from multiple antineoplastic drugs. |
Databáze: | OpenAIRE |
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