Nanoplasmonic Structure of a Polycarbonate Substrate Integrated with Parallel Microchannels for Label-Free Multiplex Detection
| Autor: | Wen Chang Kuo, Horn-Jiunn Sheen, Yi Jung Lu, Pei-Kuen Wei, Te Wei Chiu, Yu Jui Fan, Hidetoshi Tahara, Han Yun Hsieh |
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| Rok vydání: | 2021 |
| Předmět: |
Materials science
multiplex detection Polymers and Plastics Polydimethylsiloxane Spectrometer business.industry Organic chemistry General Chemistry Substrate (electronics) biosensor Article chemistry.chemical_compound QD241-441 chemistry nanoimprinting Triethoxysilane Optoelectronics Multiplex Surface plasmon resonance business surface plasmon resonance (SPR) Biosensor Refractive index |
| Zdroj: | Polymers, Vol 13, Iss 3294, p 3294 (2021) Polymers Volume 13 Issue 19 |
| ISSN: | 2073-4360 |
| Popis: | In this study, a multiplex detection system was proposed by integrating a localized surface plasmon resonance (LSPR) sensing array and parallel microfluidic channels. The LSPR sensing array was fabricated by nanoimprinting and gold sputter on a polycarbonate (PC) substrate. The polydimethylsiloxane (PDMS) microfluidic channels and PC LSPR sensing array were bound together through (3-aminopropyl)triethoxysilane (APTES) surface treatment and oxygen plasma treatment. The resonant spectrum of the LSPR sensing device was obtained by broadband white-light illumination and polarized wavelength measurements with a spectrometer. The sensitivity of the LSPR sensing device was measured using various ratios of glycerol to water solutions with different refractive indices. Multiplex detection was demonstrated using human immunoglobulin G (IgG), IgA, and IgM. The anti-IgG, anti-IgA, and anti-IgM were separately modified in each sensing region. Various concentrations of human IgG, IgA, and IgM were prepared to prove the concept that the parallel sensing device can be used to detect different targets. |
| Databáze: | OpenAIRE |
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