Isolation and elucidation of two isoflavonoids from an American Indian plant, Amorpha canescens Pursh, using Magnetic Microbead Affinity Selection Screening (MagMASS) for estrogen receptor alpha ligands

Autor:	David C. Lankin, Chun-Tao Che, Brian Guo, Birgit M. Dietz, Djaja Djendoel Soejarto, Dejan Nikolic, Jianming Ju, Tristesse Burton, Richard B. van Breemen
Rok vydání:	2021
Předmět:	biology medicine.drug_class Chemistry Cell Plant Science Microbead (research) Asteraceae biology.organism_classification Biochemistry Article In vitro medicine.anatomical_structure Isoflavonoid Estrogen medicine Amorpha canescens Agronomy and Crop Science Estrogen receptor alpha Biotechnology
Zdroj:	Phytochem Lett
ISSN:	1874-3900
DOI:	10.1016/j.phytol.2021.07.018
Popis:	A new isoflavonoid, xanthocerin J, along with previously described xanthocerin A, were isolated from a methanol extract of aerial parts of a traditional American Indian herb, Amorpha canescens Pursh (Asteraceae). The structures of these compounds were characterized using mass spectrometry and NMR based on an isolation protocol using magnetic microbead affinity selection screening (MagMASS) for ligands to the estrogen receptor alpha (ERα). These compounds bound to ERα from an active fraction that exhibited dose-dependent antiestrogenic activity in the in vitro Ishikawa assay. However, these compounds did not exhibit antiestrogenic activity in the cell-based Ishikawa assay. Xanthocerin A and J may exhibit synergistic or additive activity with other compounds found in A. canescens which needs further exploration. This work highlights the potential of A. canescens as a prospect for the future discovery of compounds for women’s health related to estrogen pathways.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7029ec8cdc06f4c50aa44d546477a95b https://doi.org/10.1016/j.phytol.2021.07.018 Zobrazit plný text záznamu Full Text from ScienceDirect