The molecular pH-response mechanism of the plant light-stress sensor PsbS
| Autor: | Anjali Pandit, Maithili Krishnan-Schmieden, Patrick E. Konold, John T. M. Kennis |
|---|---|
| Přispěvatelé: | LaserLaB - Energy, Biophysics Photosynthesis/Energy |
| Rok vydání: | 2018 |
| Předmět: |
Protein Conformation
alpha-Helical 0301 basic medicine Light Photosystem II Science Protein subunit Mutant Optical spectroscopy Biophysics Glutamic Acid General Physics and Astronomy 010402 general chemistry Physcomitrella patens Thylakoids 01 natural sciences Article General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Protein structure Stress Physiological Photosynthesis Nuclear Magnetic Resonance Biomolecular Infrared spectroscopy Multidisciplinary biology Chemistry Concerted reaction Biological techniques Mutagenesis Photosystem II Protein Complex food and beverages General Chemistry Hydrogen-Ion Concentration Molecular biophysics biology.organism_classification Adaptation Physiological Bryopsida Recombinant Proteins 0104 chemical sciences Folding (chemistry) 030104 developmental biology Thylakoid Photoprotection Mutagenesis Site-Directed SDG 6 - Clean Water and Sanitation |
| Zdroj: | Nature Communications, Vol 12, Iss 1, Pp 1-11 (2021) Nature Communications, 12(1):2291, 1-11. Nature Publishing Group Nature Communications, 12, 2291 Krishnan-Schmieden, M, Konold, P E, Kennis, J T M & Pandit, A 2021, ' The molecular pH-response mechanism of the plant light-stress sensor PsbS ', Nature Communications, vol. 12, no. 1, 2291, pp. 1-11 . https://doi.org/10.1038/s41467-021-22530-4 Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1101/457614 |
| Popis: | Plants need to protect themselves from excess light, which causes photo-oxidative damage and lowers the efficiency of photosynthesis. Photosystem II subunit S (PsbS) is a pH sensor protein that plays a crucial role in plant photoprotection by detecting thylakoid lumen acidification in excess light conditions via two lumen-faced glutamates. However, how PsbS is activated under low-pH conditions is unknown. To reveal the molecular response of PsbS to low pH, here we perform an NMR, FTIR and 2DIR spectroscopic analysis of Physcomitrella patens PsbS and of the E176Q mutant in which an active glutamate has been replaced. The PsbS response mechanism at low pH involves the concerted action of repositioning of a short amphipathic helix containing E176 facing the lumen and folding of the luminal loop fragment adjacent to E71 to a 310-helix, providing clear evidence of a conformational pH switch. We propose that this concerted mechanism is a shared motif of proteins of the light-harvesting family that may control thylakoid inter-protein interactions driving photoregulatory responses. Photosystem II subunit S (PsbS) senses thylakoid lumen acidification when plants are exposed to excess light. Here the authors use NMR and IR spectroscopy to show that low pH causes repositioning of an amphipathic helix and folding of a loop involving critical pH sensing glutamate residues in PsbS. |
| Databáze: | OpenAIRE |
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