In situ localization of gelatinolytic activity during development and resorption of Meckel's cartilage in mice
| Autor: | Yasunori Sakakura, Yoichiro Hosokawa, Eichi Tsuruga, Kazuharu Irie, Toshihiko Yajima |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Pathology
medicine.medical_specialty Mice Inbred Strains Mandible Matrix metalloproteinase Matrix (biology) Immunoenzyme Techniques Extracellular matrix Mice Calcification Physiologic Chondrocytes Matrix Metalloproteinase 13 medicine Animals Perichondrium General Dentistry Fluorescent Dyes Chemistry Cartilage medicine.disease Extracellular Matrix Resorption Cell biology medicine.anatomical_structure Gelatinases embryonic structures Electrophoresis Polyacrylamide Gel Proteoglycans Meckel's cartilage Calcification |
| Zdroj: | European Journal of Oral Sciences. 115:212-223 |
| ISSN: | 1600-0722 0909-8836 |
| Popis: | Degradation of Meckel's cartilage in the middle portion is accompanied by hypertrophy and death of chondrocytes, calcification of the cartilaginous matrix, and chondroclastic resorption. We hypothesize that the gelatinolytic activity of matrix metalloproteinases (MMPs) largely contributes to the degradation of extracellular matrix (ECM) in the process. The activity in Meckel's cartilage of mouse mandibular arches at embryonic days 14-16 (E14-E16) was examined by a combination of in situ zymography (ISZ), using quenched fluorescent dye-labeled gelatin as a substrate, with CTT (a selective inhibitor of MMP-2 and -9) or with EDTA (a general MMP inhibitor). On E14 and E15, ISZ showed fluorescence in the perichondrium, in the intercellular septa between chondrocytes, and in the nucleus of chondrocytes. CTT attenuated fluorescence, and EDTA eliminated it. On E16, calcified cartilaginous matrix showed intense fluorescence, and dot-like fluorescence was observed in as-yet uncalcified intercellular septa, even after CTT treatment. EDTA inhibited fluorescence, but unexpectedly intense fluorescence was found in the cytoplasm of hypertrophic chondrocytes facing the resorption front. MMP-2, -9, and -13 immunoreactivity was detected in the perichondrium and chondrocytes of Meckel's cartilage. These findings suggest that MMPs and other proteinases capable of degrading gelatin play an integral role in the development, calcification, and resorption of Meckel's cartilage through ECM reconstitution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text