Ellipsoidal Polyaspartamide Polymersomes with Enhanced Cell-Targeting Ability
| Autor: | Christopher Brockman, Hyunjoon Kong, Charles M. Schroeder, Jae Hyun Jeong, Mei Hsiu Lai, Ross J. DeVolder |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Materials science Nanoparticle Nanotechnology Polymer Condensed Matter Physics Article Electronic Optical and Magnetic Materials Biomaterials chemistry.chemical_compound chemistry Polymersome PEG ratio Electrochemistry Biophysics Nanobiotechnology Self-assembly Cell adhesion Ethylene glycol |
| Popis: | Nano-sized polymersomes functionalized with peptides or proteins are being increasingly studied for targeted delivery of diagnostic and therapeutic molecules. Earlier computational studies have suggested that ellipsoidal nanoparticles, compared to spherical ones, display enhanced binding efficiency with target cells, but this has not yet been experimentally validated. We hypothesize that hydrophilic polymer chains coupled to vesicle-forming polymers would result in ellipsoidal polymersomes. In addition, ellipsoidal polymersomes modified with cell adhesion peptides bind with target cells more actively than spherical ones. We examine this hypothesis by substituting polyaspartamide with octadecyl chains and varying numbers of poly(ethylene glycol) (PEG) chains. Increasing the degree of substitution of PEG from 0.5 to 1.0 mol% drives the polymer to self-assemble into an ellipsoidal polymersome with an aspect ratio of 2.1. Further modification of these ellipsoidal polymersomes with peptides containing an Arg-Gly-Asp sequence (RGD peptides) lead to a significant increase in the rate of association and decrease in the rate of dissociation with a substrate coated with αvβ3 integrins. In addition, in a circulation-mimicking flow, the ellipsoidal polymersomes linked with RGD peptides adhere to target tissues more favorably than their spherical equivalents do. Overall, the results of this study will greatly serve to improve the efficiency of targeted delivery of a wide array of polymersomes loaded with various biomedical modalities. |
| Databáze: | OpenAIRE |
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