Salinity Stress Enhances the Antioxidant Capacity of Bacillus and Planococcus Species Isolated From Saline Lake Environment
| Autor: | Abdelrahim H.A. Hassan, Hamada AbdElgawad, Gerrit T.S. Beemster, Ahmed S. M. Mousa, Dalal Hussien M. Alkhalifah, Sulaiman A. Al Yousef, Wael N. Hozzein |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Antioxidant medicine.medical_treatment lcsh:QR1-502 Bacillus medicine.disease_cause Microbiology lcsh:Microbiology Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound medicine stress markers Food science Biology 030304 developmental biology Original Research salinity stress Planococcus 0303 health sciences biology 030306 microbiology Chemistry Glutathione biology.organism_classification antioxidants Catalase biology.protein Oxidative stress Bacteria Peroxidase |
| Zdroj: | Frontiers in Microbiology Frontiers in microbiology Frontiers in Microbiology, Vol 11 (2020) |
| ISSN: | 1664-302X |
| Popis: | This study aims at exploiting salinity stress as an innovative, simple, and cheap method to enhance the production of antioxidant metabolites and enzymes from bacteria for potential application as functional additives to foods and pharmaceuticals. We investigated the physiological and biochemical responses of four bacterial isolates, which exhibited high tolerance to 20% NaCl (wt/vol), out of 27 bacterial strains isolated from Aushazia Lake, Qassim region, Saudi Arabia. The phylogenetic analysis of the 16S rRNA genes of these four isolates indicated that strains ST1 and ST2 belong to genusBacillus, whereas strains ST3 and ST4 belong to genusPlanococcus. Salinity stress differentially induced oxidative damage, where strains ST3 and ST4 showed increased lipid peroxidation, lipoxygenase, and xanthine oxidase levels. Consequently, high antioxidant contents were produced to control oxidative stress, particularly in ST3 and ST4. These twoPlanococcusstrains showed increased glutathione cycle, phenols, flavonoids, antioxidant capacity, catalase, and/or superoxide dismutase (SOD). Interestingly, the production of glutathione byPlanococcusstrains was some thousand folds greater than by higher plants. On the other hand, the induction of antioxidants in ST1 and ST2 was restricted to phenols, flavonoids, peroxidase, glutaredoxin, and/or SOD. The hierarchical analysis also supported strain-specific responses. This is the first report that exploited salinity stress for promoting the production of antioxidants from bacterial isolates, which can be utilized as postbiotics for promising applications in foods and pharmaceuticals. |
| Databáze: | OpenAIRE |
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