FGF-2 Regulates Enamel and Dentine Formation in Mouse Tooth Germ
| Autor: | T. Tsuboi, Akifumi Togari, S. Mizutani, Koji Hirukawa, M. Nakano |
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| Rok vydání: | 2003 |
| Předmět: |
Sialoglycoproteins
Endocrinology Diabetes and Metabolism Cellular differentiation Basic fibroblast growth factor Mice Inbred Strains Biology Fibroblast growth factor Extracellular matrix Mice chemistry.chemical_compound Organ Culture Techniques Endocrinology Dental Enamel Proteins stomatognathic system Pregnancy Gene expression Animals Orthopedics and Sports Medicine RNA Messenger Protein Precursors Dental Enamel Extracellular Matrix Proteins Amelogenin Reverse Transcriptase Polymerase Chain Reaction Cell growth Gene Expression Regulation Developmental Tooth Germ Oligonucleotides Antisense Alkaline Phosphatase Phosphoproteins Molar Molecular biology stomatognathic diseases chemistry Dentin Odontogenesis Alkaline phosphatase Female Fibroblast Growth Factor 2 Biomarkers |
| Zdroj: | Calcified Tissue International. 73:496-501 |
| ISSN: | 1432-0827 0171-967X |
| Popis: | We examined the effects of basic fibroblast growth factor (FGF-2) on cultured lower molar tooth germ at the differentiative (bell) stage. Although FGF-2 has been detected in odontogenesis, its roles in biological activities, such as cell proliferation, differentiation and extracellular matrix mineralization are unclear. We assayed mRNA levels of the differentiation markers, dentine sialophosphoprotein (DSPP), amelogenin and alkaline phosphatase (ALP) using reverse transcription-polymerase chain reaction (RT-PCR), and histological methods. Tooth germs dissected from 17-day-old embryonic mice were cultured for 4 days with either recombinant human FGF-2 or specific antisense phosphorothioate oligodeoxynucleotide (antisense ODN) for FGF-2. Exogenous FGF-2 decreased the gene expression of differentiation markers in molars at the bell stage. Abrogation of endogenous FGF-2 by antisense ODN increased the gene expression of differentiation markers, and also significantly enhanced enamel and dentine formation. This histological change was recovered by adding exogeneous FGF-2. These findings suggest that FGF-2 at the bell stage regulates cell differentiation and matrix secretion. |
| Databáze: | OpenAIRE |
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