Determination of guanase activity in normal and pathological sera by high-performance liquid chromatography
| Autor: | E. Cernia, Paola Castellano, Anna Maria Girelli, A. Bozza, Silvia Canepari |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Clinical Biochemistry Reference range Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Hplc assay Guanase activity Drug Discovery Humans In patient Molecular Biology Pathological Chromatography High Pressure Liquid Guanine Deaminase Pharmacology Chromatography Liver Diseases Mean value Reproducibility of Results General Medicine Xanthine chemistry Female Spectrophotometry Ultraviolet |
| Zdroj: | Biomedical Chromatography. 9:130-134 |
| ISSN: | 1099-0801 0269-3879 |
| DOI: | 10.1002/bmc.1130090304 |
| Popis: | A simple HPLC assay for serum guanase based on the direct determination of enzymatically formed xanthine was applied to normal and pathological sera. The procedure is sensitive, precise (CV below 5%) and suitable for routine purposes, and the method requires only 50 microL of sample. Using this method the reference range as determined from the sera of 40 healthy adult controls is 0-1.1 U/L. In patients with various liver diseases serum guanase activities were found to be increased 5- to 50-fold compared with the normal mean value. |
| Databáze: | OpenAIRE |
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