Monitoring excitatory amino acid release in vivo by microdialysis with capillary electrophoresis-electrochemistry
| Autor: | Brad P. Bammel, Susan M. Lunte, Craig E. Lunte, Paul L. Weber, Malcolm R. Smyth, Thomas J. O'Shea |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Electrophoresis
Male Microdialysis Biochemistry Analytical Chemistry Rats Sprague-Dawley Capillary electrophoresis Electrochemistry Animals Amino Acids Voltammetry Detection limit Alanine chemistry.chemical_classification Brain Chemistry Chromatography Chemistry Organic Chemistry Glutamate receptor General Medicine Amino acid Rats Excitatory postsynaptic potential Potassium Indicators and Reagents Dialysis |
| Zdroj: | Journal of chromatography. 608(1-2) |
| Popis: | Capillary electrophoresis (CE) with electrochemical detection (ED) was used to determine extracellular levels of aspartate, glutamate and alanine in samples from the frontoparietal cortex of the rat which were obtained by microdialysis. The method was used to monitor the effect on the overflow of the excitatory amino acids aspartate and glutamate of an influx of high concentrations of potassium ion. Samples were derivatized with naphthalenedialdehyde-cyanide prior to analysis. Detection limits for aspartate and glutamate were 80 and 100 nM, respectively. CEED is extremely useful for the analysis of microdialysis samples because of the very small sample volumes required by this analytical technique. The use of ED provides the requisite sensitivity and allows verification of peak purity by voltammetry. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|