Binding and internalization of leptin by porcine choroid plexus cells in culture
| Autor: | Christian Peiser, Rudolf E. Lang, Gerard P. McGregor |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Leptin
medicine.medical_specialty Swine media_common.quotation_subject Receptors Cell Surface Biology Iodine Radioisotopes Radioligand Assay chemistry.chemical_compound Cerebrospinal fluid Adipocyte Internal medicine medicine Animals Internalization Intracellular part Cells Cultured media_common Plexus Leptin receptor General Neuroscience digestive oral and skin physiology Biological Transport Epithelial Cells Endocrinology chemistry Choroid Plexus Receptors Leptin Choroid plexus Carrier Proteins hormones hormone substitutes and hormone antagonists Protein Binding |
| Zdroj: | Neuroscience Letters. 283:209-212 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/s0304-3940(00)00942-3 |
| Popis: | Leptin is an adipocyte derived hormone with profound behavioural and metabolic effects exerted by both central and peripheral sites of action. One of its targets in the central nervous system appears to be the epithelial cells of the choroid plexus where leptin receptor (OB-R) expression is particularly high. The most abundant receptor subtype at this site is OB-Ra which is truncated at its intracellular part and has been suggested to serve functions such as leptin transport or clearance. The choroid plexus may thus be a site where receptor mediated exchange of leptin between cerebrospinal fluid and blood takes place. The study here shows that porcine plexus epithelia preserve their ability of OB-R expression when grown in culture. In addition, our experiments suggest that leptin is rapidly internalized upon binding to these cells supporting the view of an OB-R mediated transport of leptin across the choroid plexus. |
| Databáze: | OpenAIRE |
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