Neoplastic transformation of hamster embryo cells by heavy ions
| Autor: | M Ikenaga, Yoshiya Furusawa, Masao Suzuki, Fumio Suzuki, Hiroyuki Suzuki, Tomohisa Kato, Zhen Bo Han |
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| Rok vydání: | 1998 |
| Předmět: |
Silicon
Atmospheric Science Cell Survival Aerospace Engineering Hamster Linear energy transfer Radiation Dosage Ion Cricetinae Relative biological effectiveness Animals Heavy Ions Linear Energy Transfer Neoplastic transformation Irradiation Cell Line Transformed Mesocricetus biology Chemistry X-Rays Radiochemistry Dose-Response Relationship Radiation Astronomy and Astrophysics biology.organism_classification Carbon Cell Transformation Neoplastic Geophysics Space and Planetary Science Cell culture General Earth and Planetary Sciences Atomic physics Relative Biological Effectiveness Synchrotrons |
| Zdroj: | Advances in Space Research. 22:1725-1732 |
| ISSN: | 0273-1177 |
| DOI: | 10.1016/s0273-1177(99)00038-1 |
| Popis: | We have studied the induction of morphological transformation of Syrian hamster embryo cells by low doses of heavy ions with different linear energy transfer (LET), ranging from 13 to 400 keV/micrometer. Exponentially growing cells were irradiated with 12C or 28Si ion beams generated by the Heavy Ion Medical Accelerator in Chiba (HIMAC), inoculated to culture dishes, and transformed colonies were identified when the cells were densely stacked and showed a crisscross pattern. Over the LET range examined, the frequency of transformation induced by the heavy ions increased sharply at very low doses no greater than 5 cGy. The relative biological effectiveness (RBE) of the heavy ions relative to 250 kVp X-rays showed an initial increase with LET, reaching a maximum value of about 7 at 100 keV/micrometer, and then decreased with the further increase in LET. Thus, we confirmed that high LET heavy ions are significantly more effective than X-rays for the induction of in vitro cell transformation. |
| Databáze: | OpenAIRE |
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