Effect of 5-aminolevulinic acid photodynamic therapy on keratinocyte proliferation and apoptosis in condyloma acuminatum
| Autor: | Guangwen Yin, Ke Sha, Xuyang Li, Xianxian Xia, Xinxin Pan, Bingjie Cai, Fangfang Li |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Keratinocytes 0301 basic medicine 030103 biophysics Adolescent Proliferation index medicine.medical_treatment Biophysics Apoptosis Photodynamic therapy Dermatology Young Adult 03 medical and health sciences 0302 clinical medicine medicine Humans Pharmacology (medical) Cell Proliferation Photosensitizing Agents Dose-Response Relationship Drug biology Dose-Response Relationship Radiation Aminolevulinic Acid Middle Aged Condyloma Acuminatum Treatment Outcome medicine.anatomical_structure Photochemotherapy Oncology Condylomata Acuminata 030220 oncology & carcinogenesis Ki-67 Immunology Cancer research biology.protein Immunohistochemistry Keratinocyte After treatment |
| Zdroj: | Photodiagnosis and Photodynamic Therapy. 18:310-314 |
| ISSN: | 1572-1000 |
| DOI: | 10.1016/j.pdpdt.2017.03.003 |
| Popis: | Background The effect of 5-aminolevulinic acid photodynamic therapy on keratinocyte proliferation and apoptosis in condyloma acuminatum tissues was evaluated. Methods An immunohistochemical method and TdT-mediated dUTP nick end labeling were performed to detect the positive expression of the keratinocyte proliferation-related gene Ki-67 and apoptosis, respectively, in condyloma acuminatum tissues. Results Of 52 cases, 44 showed positive expression of Ki-67 in condyloma acuminatum keratinocytes before the treatment, with a positive expression rate of 84.62% (44/52), an expression strength of mostly ++ − +++, and a Ki-67 proliferation index of 80.26 ± 5.07%. After treatment, 22 cases showed positive expression of Ki-67 in condyloma acuminatum keratinocytes, with a positive expression rate of 42.31% (22/52), an expression strength of mostly − − ++, and a proliferation index of 42.67 ± 3.06%. The differences in the positive expression rate, expression strength, and proliferation index in the before- and after-treatment groups were statistically significant (χ2 = 20.070, P Conclusion 5-Aminolevulinic acid photodynamic therapy can inhibit the proliferation and promote the apoptosis of keratinocytes, and represents an effective mechanism for treating condyloma acuminatum. |
| Databáze: | OpenAIRE |
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