Lateral Flow Genochromatographic Strip for Naked-Eye Detection of Mycobacterium Tuberculosis PCR Products with Gold Nanoparticles as a Reporter
| Autor: | Naghmeh Sattarahmady, Tondro G H, Hossein Heli, Haghkhah M, Dehdari Vais R, R. Nazari-Vanani |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Streptavidin
nucleic acid hybridization lcsh:Medical physics. Medical radiology. Nuclear medicine lcsh:R895-920 01 natural sciences law.invention Mycobacterium tuberculosis Lateral flow test 03 medical and health sciences chemistry.chemical_compound law metal nanoparticles Polymerase chain reaction 030304 developmental biology 0303 health sciences Mycobacterium bovis biology 010401 analytical chemistry gold biology.organism_classification Molecular biology 0104 chemical sciences test strip chemistry lateral flow tuberculosis Colloidal gold Biotinylation Conjugate |
| Zdroj: | Journal of Biomedical Physics and Engineering, Vol 10, Iss 3, Pp 307-318 (2020) |
| ISSN: | 2251-7200 |
| Popis: | Background: Mycobacterium tuberculosis (MTB) is a pathogen causing tuberculosis (TB) in human, and TB can cause enormous social and economic disruptions. Lateral flow test strips (LFTSs) are inexpensive, portable, disposable, rapid, and easy-to-use analytical tools. Objective: LFTSs were prepared for the detection of MTB. LFTSs were fabricated using a new specific probe for MTB H37Rv, based on IS6110 sequence gene, and tailed with poly deoxyadenine (dA).Material and Methods: In this experimental study, to create test and control zones, streptavidin (STP) and a 150-mer dA were dotted on a nitrocellolose membrane. Gold nanoparticles (GNPs) were conjugated with poly deoxythymidine sequence and placed on the conjugate pad. The composition of immersion buffers for sample pad and conjugate pad, running solution, solutions of GNPs-S-dT150 and STP were introduced. DNA genome of MTB and Mycobacterium bovis in clinical samples was amplified with PCR, and then detected by the LFTSs. During the assay, samples were firstly hybridized in two steps and then placed on a conjugate pad in a manner that positive and negative samples provided two and one red lines, respectively, on the detection pad. Results: After PCR reaction with biotinylated primer, hybridization process with specific MTB probe-dA70-100 toke 10 min, and running process on the strip was performed within 5 min. Conclusion: We showed that LFTS can discriminate a particular bacteria strain from others. The LFTSs can be redesigned for detection of other pathogenic genomes. |
| Databáze: | OpenAIRE |
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