Deletion, mutation, and loss of expression of KLF6 in human prostate cancer
| Autor: | Robert L. Vessella, Ceshi Chen, Xiaodong Sun, Heikki Helin, Jin-Tang Dong, E. Hyytinen, Henry F. Frierson, Pasi A. Koivisto |
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| Rok vydání: | 2003 |
| Předmět: |
Male
Tumor suppressor gene Transplantation Heterologous Kruppel-Like Transcription Factors Loss of Heterozygosity Biology Severe Acute Respiratory Syndrome medicine.disease_cause Pathology and Forensic Medicine Loss of heterozygosity Prostate cancer Mice Prostate Proto-Oncogene Proteins medicine Kruppel-Like Factor 6 Tumor Cells Cultured Animals Humans Genes Tumor Suppressor DNA Primers Base Sequence Reverse Transcriptase Polymerase Chain Reaction Cancer Prostatic Neoplasms Zinc Fingers Exons medicine.disease United States DNA-Binding Proteins Repressor Proteins Gene Expression Regulation Neoplastic medicine.anatomical_structure KLF6 Severe acute respiratory syndrome-related coronavirus Mutation Cancer research Trans-Activators Commentary Adenocarcinoma Carcinogenesis 5' Untranslated Regions Cell Division Gene Deletion Regular Articles Transcription Factors |
| Zdroj: | The American journal of pathology. 162(4) |
| ISSN: | 0002-9440 |
| Popis: | Kruppel-like factors (KLFs) are a group of transcription factors that appear to be involved in different biological processes including carcinogenesis. In a recent study, KLF6 was reported as a tumor suppressor gene in prostate cancer because of its frequent loss of heterozygosity (LOH) and mutation as well as functional suppression of cell proliferation. Loss of chromosomal locus spanning KLF6 is relatively infrequent in other published studies of prostate cancer, however. To clarify the role of KLF6 in prostate cancers, particularly those that are high grade, we examined KLF6 for deletion, mutation, and loss of expression in 96 prostate cancer samples including 21 xenografts/cell lines. Loss of heterozygosity occurred in 4 (19%) of 21 xenografts/cell lines and 8 (28%) of 29 informative tumors. Fourteen of the 96 (15%) samples showed 15 somatic sequence changes in the KLF6 gene, including 7 that changed KLF6 peptide sequences, 4 that did not, and 4 that were located in untranslated regions. Expression levels of KLF6 were significantly lost in 4 of 20 (20%) xenografts/cell lines of prostate cancer, as detected by RT-PCR and Northern blot analysis. These findings indicate that significant genetic alterations of KLF6 occur in a minority of high-grade prostate cancers. |
| Databáze: | OpenAIRE |
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